• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

中性粒细胞损害视网膜色素上皮屏障的完整性。

Neutrophils compromise retinal pigment epithelial barrier integrity.

作者信息

Zhou Jiehao, He Shikun, Zhang Ning, Spee Christine, Zhou Peng, Ryan Stephen J, Kannan Ram, Hinton David R

机构信息

The Arnold and Mabel Beckman Macular Research Center at the Doheny Eye Institute, University of Southern California, Los Angeles, CA 90033, USA.

出版信息

J Biomed Biotechnol. 2010;2010:289360. doi: 10.1155/2010/289360. Epub 2010 Mar 3.

DOI:10.1155/2010/289360
PMID:20204129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2831460/
Abstract

We hypothesized that neutrophils and their secreted factors mediate breakdown of the integrity of the outer blood-retina-barrier by degrading the apical tight junctions of the retinal pigment epithelium (RPE). The effect of activated neutrophils or neutrophil cell lysate on apparent permeability of bovine RPE-Choroid explants was evaluated by measuring [3H] mannitol flux in a modified Ussing chamber. The expression of matrix metalloproteinase- (MMP-) 9 in murine peritoneal neutrophils, and the effects of neutrophils on RPE tight-junction protein expression were assessed by confocal microscopy and western blot. Our results revealed that basolateral incubation of explants with neutrophils decreased occludin and ZO-1 expression at 1 and 3 hours and increased the permeability of bovine RPE-Choroid explants by >3-fold (P < .05). Similarly, basolateral incubation of explants with neutrophil lysate decreased ZO-1 expression at 1 and 3 hours (P < .05) and increased permeability of explants by 75%. Further, we found that neutrophils prominently express MMP-9 and that incubation of explants with neutrophils in the presence of anti-MMP-9 antibody inhibited the increase in permeability. These data suggest that neutrophil-derived MMP-9 may play an important role in disrupting the integrity of the outer blood-retina barrier.

摘要

我们推测，中性粒细胞及其分泌因子通过降解视网膜色素上皮（RPE）的顶端紧密连接，介导外血视网膜屏障完整性的破坏。通过在改良的Ussing室中测量[3H]甘露醇通量，评估活化的中性粒细胞或中性粒细胞裂解物对牛RPE - 脉络膜外植体表观通透性的影响。通过共聚焦显微镜和蛋白质印迹法评估基质金属蛋白酶-（MMP-）9在小鼠腹腔中性粒细胞中的表达，以及中性粒细胞对RPE紧密连接蛋白表达的影响。我们的结果显示，外植体与中性粒细胞在基底外侧孵育1小时和3小时后，闭合蛋白和ZO-1表达降低，牛RPE - 脉络膜外植体的通透性增加超过3倍（P <.05）。同样，外植体与中性粒细胞裂解物在基底外侧孵育1小时和3小时后，ZO-1表达降低（P <.05），外植体通透性增加75％。此外，我们发现中性粒细胞显著表达MMP-9，并且在抗MMP-9抗体存在下，外植体与中性粒细胞孵育可抑制通透性增加。这些数据表明，中性粒细胞衍生的MMP-9可能在破坏外血视网膜屏障的完整性中起重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/51a04460b386/JBB2010-289360.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/a912877677e3/JBB2010-289360.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/7a7c2df59215/JBB2010-289360.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/b5febc48622c/JBB2010-289360.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/2dabadb02de1/JBB2010-289360.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/59bd49469261/JBB2010-289360.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/be36876ef4ea/JBB2010-289360.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/51a04460b386/JBB2010-289360.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/a912877677e3/JBB2010-289360.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/7a7c2df59215/JBB2010-289360.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/b5febc48622c/JBB2010-289360.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/2dabadb02de1/JBB2010-289360.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/59bd49469261/JBB2010-289360.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/be36876ef4ea/JBB2010-289360.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cd5/2831460/51a04460b386/JBB2010-289360.007.jpg

相似文献

1
Neutrophils compromise retinal pigment epithelial barrier integrity.中性粒细胞损害视网膜色素上皮屏障的完整性。
J Biomed Biotechnol. 2010;2010:289360. doi: 10.1155/2010/289360. Epub 2010 Mar 3.
2
Remodeling of junctional complexes during the development of the outer blood-retinal barrier.外血视网膜屏障发育过程中连接复合体的重塑。
Anat Rec. 1997 Nov;249(3):380-8. doi: 10.1002/(SICI)1097-0185(199711)249:3<380::AID-AR9>3.0.CO;2-Y.
3
Characterization of brimonidine transport in retinal pigment epithelium.视网膜色素上皮细胞中溴莫尼定转运的特性研究
Invest Ophthalmol Vis Sci. 2006 Jan;47(1):287-94. doi: 10.1167/iovs.05-0189.
4
Regulation of RPE intercellular junction integrity and function by hepatocyte growth factor.肝细胞生长因子对视网膜色素上皮细胞间连接完整性和功能的调节
Invest Ophthalmol Vis Sci. 2002 Aug;43(8):2782-90.
5
Effects of high glucose concentration on the barrier function and the expression of tight junction proteins in human retinal pigment epithelial cells.高葡萄糖浓度对人视网膜色素上皮细胞屏障功能和紧密连接蛋白表达的影响。
Exp Eye Res. 2009 Dec;89(6):913-20. doi: 10.1016/j.exer.2009.07.017. Epub 2009 Aug 4.
6
Vectorial release of matrix metalloproteinases (MMPs) from porcine RPE-choroid explants following selective retina therapy (SRT): towards slowing the macular ageing process.猪眼视网膜色素上皮-脉络膜组织块经选择性视网膜光凝治疗（SRT）后 MMP 的定向释放：延缓黄斑老化进程。
Exp Eye Res. 2012 Apr;97(1):63-72. doi: 10.1016/j.exer.2012.02.011. Epub 2012 Feb 22.
7
Apical and basal regulation of the permeability of the retinal pigment epithelium.视网膜色素上皮通透性的顶端和基底调节
Invest Ophthalmol Vis Sci. 2003 Feb;44(2):808-17. doi: 10.1167/iovs.02-0473.
8
Effect of retinoic acid on the tight junctions of the retinal pigment epithelium-choroid complex of guinea pigs with lens-induced myopia in vivo.视黄酸对体内诱导性近视豚鼠视网膜色素上皮-脉络膜复合体紧密连接的影响。
Int J Mol Med. 2014 Apr;33(4):825-32. doi: 10.3892/ijmm.2014.1651. Epub 2014 Feb 10.
9
Intracellular amyloid beta alters the tight junction of retinal pigment epithelium in 5XFAD mice.细胞内淀粉样蛋白β改变5XFAD小鼠视网膜色素上皮的紧密连接。
Neurobiol Aging. 2014 Sep;35(9):2013-20. doi: 10.1016/j.neurobiolaging.2014.03.008. Epub 2014 Mar 15.
10
High glucose concentration leads to differential expression of tight junction proteins in human retinal pigment epithelial cells.高葡萄糖浓度导致人视网膜色素上皮细胞中紧密连接蛋白的差异表达。
Endocrinol Nutr. 2009 Feb;56(2):53-8. doi: 10.1016/S1575-0922(09)70552-2. Epub 2009 May 1.

引用本文的文献

1
Benefit of nanohydroxyapatite combined with triamcinolone for non-surgical treatment of severe periodontitis: a retrospective study.纳米羟基磷灰石联合曲安奈德非手术治疗重度牙周炎的疗效：一项回顾性研究
Am J Transl Res. 2025 Jun 15;17(6):4663-4678. doi: 10.62347/WGSE9643. eCollection 2025.
2
Polarized RPE Secretome Preserves Photoreceptors in Retinal Dystrophic RCS Rats.极化的 RPE 分泌组可保留 RCS 大鼠视网膜变性中的感光细胞。
Cells. 2023 Jun 22;12(13):1689. doi: 10.3390/cells12131689.
3
Correlation between ultrawide-field fluorescence contrast results and white blood cell indexes in diabetic retinopathy.

本文引用的文献

1
Effects of crystalline glucocorticoid triamcinolone acetonide on cultered human supraspinatus tendon cells.曲安奈德（一种晶状糖皮质激素）对体外培养的人冈上肌腱细胞的影响。
Acta Orthop. 2009 Jun;80(3):357-62. doi: 10.3109/17453670902988360.
2
A protocol for the culture and differentiation of highly polarized human retinal pigment epithelial cells.一种高度极化的人视网膜色素上皮细胞培养与分化方案。
Nat Protoc. 2009;4(5):662-73. doi: 10.1038/nprot.2009.33.
3
Mechanisms of leukocyte migration across the blood-retina barrier.白细胞穿越血视网膜屏障的机制。
糖尿病视网膜病变中超宽视野荧光对比结果与白细胞指标的相关性。
BMC Ophthalmol. 2022 May 21;22(1):231. doi: 10.1186/s12886-022-02442-7.
4
Structural abnormalities of retinal pigment epithelial cells in a light-inducible, rhodopsin mutant mouse.光诱导视紫红质突变小鼠视网膜色素上皮细胞的结构异常。
J Anat. 2023 Aug;243(2):223-234. doi: 10.1111/joa.13667. Epub 2022 Apr 15.
5
Retinal Pigment Epithelium Remodeling in Mouse Models of Retinitis Pigmentosa.视网膜色素上皮重塑在视网膜色素变性的小鼠模型中。
Int J Mol Sci. 2021 May 20;22(10):5381. doi: 10.3390/ijms22105381.
6
Potential Role of Myeloid-Derived Suppressor Cells (MDSCs) in Age-Related Macular Degeneration (AMD).髓系来源的抑制细胞（MDSCs）在年龄相关性黄斑变性（AMD）中的潜在作用。
Front Immunol. 2020 Mar 20;11:384. doi: 10.3389/fimmu.2020.00384. eCollection 2020.
7
Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties.具有快速分化和改善屏障特性的视网膜色素上皮细胞系
Pharmaceutics. 2019 Aug 13;11(8):412. doi: 10.3390/pharmaceutics11080412.
8
Dynamic Interplay of Innate and Adaptive Immunity During Sterile Retinal Inflammation: Insights From the Transcriptome.无菌性视网膜炎症期间先天性免疫与适应性免疫的动态相互作用：转录组学的见解
Front Immunol. 2018 Jul 18;9:1666. doi: 10.3389/fimmu.2018.01666. eCollection 2018.
9
Extended Pharmacokinetic Model of the Rabbit Eye for Intravitreal and Intracameral Injections of Macromolecules: Quantitative Analysis of Anterior and Posterior Elimination Pathways.兔眼玻璃体内和前房内注射大分子的扩展药代动力学模型：前、后消除途径的定量分析。
Pharm Res. 2018 May 31;35(8):153. doi: 10.1007/s11095-018-2435-0.
10
Alterations in Circulating Immune Cells in Neovascular Age-Related Macular Degeneration.新生血管性年龄相关性黄斑变性患者循环免疫细胞的改变
Sci Rep. 2015 Nov 17;5:16754. doi: 10.1038/srep16754.
Semin Immunopathol. 2008 Apr;30(2):165-77. doi: 10.1007/s00281-008-0106-7. Epub 2008 Feb 28.
4
Effect of proinflammatory cytokines, tumor necrosis factor-alpha and interferon-gamma on epithelial barrier function and matrix metalloproteinase-9 in Madin Darby canine kidney cells.促炎细胞因子、肿瘤坏死因子-α和干扰素-γ对Madin Darby犬肾细胞上皮屏障功能及基质金属蛋白酶-9的影响
Cell Physiol Biochem. 2007;19(1-4):99-112. doi: 10.1159/000099198.
5
MMP-2 and MMP-9 secretion by rpe is stimulated by angiogenic molecules found in choroidal neovascular membranes.脉络膜新生血管膜中发现的血管生成分子可刺激视网膜色素上皮（RPE）分泌基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）。
Retina. 2006 Apr;26(4):454-61. doi: 10.1097/01.iae.0000238549.74626.33.
6
Interleukin (IL)-6, interleukin (IL)-8 levels and cellular composition of the vitreous humor in proliferative diabetic retinopathy, proliferative vitreoretinopathy, and traumatic proliferative vitreoretinopathy.增殖性糖尿病视网膜病变、增殖性玻璃体视网膜病变和外伤性增殖性玻璃体视网膜病变中玻璃体腔的白细胞介素（IL）-6、白细胞介素（IL）-8水平及细胞组成
Ocul Immunol Inflamm. 2005 Sep-Oct;13(5):375-81. doi: 10.1080/09273940490518900.
7
Characterization of brimonidine transport in retinal pigment epithelium.视网膜色素上皮细胞中溴莫尼定转运的特性研究
Invest Ophthalmol Vis Sci. 2006 Jan;47(1):287-94. doi: 10.1167/iovs.05-0189.
8
Ischemia injury alters endothelial cell properties of kidney cortex: stimulation of MMP-9.缺血损伤改变肾皮质内皮细胞特性：刺激基质金属蛋白酶-9。
Exp Cell Res. 2005 Oct 15;310(1):105-16. doi: 10.1016/j.yexcr.2005.07.004.
9
Intravitreal triamcinolone acetonide for treatment of intraocular proliferative, exudative, and neovascular diseases.玻璃体内注射曲安奈德治疗眼内增殖性、渗出性和新生血管性疾病。
Prog Retin Eye Res. 2005 Sep;24(5):587-611. doi: 10.1016/j.preteyeres.2005.01.004. Epub 2005 Mar 29.
10
Neutrophils promote experimental choroidal neovascularization.中性粒细胞促进实验性脉络膜新生血管形成。
Mol Vis. 2005 Jun 16;11:414-24.