黑腹果蝇中矮胖基因不等交换的速率。
The rate of unequal crossing over in the dumpy gene from Drosophila melanogaster.
机构信息
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.
出版信息
J Mol Evol. 2010 Mar;70(3):260-5. doi: 10.1007/s00239-010-9327-1. Epub 2010 Mar 5.
The PIGSFEAST (PF) exon of the Drosophila dumpy gene is undergoing concerted evolution by the process of unequal crossing over. We have developed a long-range PCR-based assay to amplify the approximately 12 kb long exon which contains variable numbers of 303 or 306 nt long repeats in a tandem array. We applied this procedure to mutation accumulation lines of Drosophila melanogaster established by M. Wayne and L. Higgins. Nine new repeat length variants were found in these lines allowing us to measure the rate of unequal crossing over in the PF exon. The rate, which for several reasons is an underestimate, is 7.05 x 10(-4) exchanges per generation.
果蝇 dumpty 基因的 PIGSFEAST（PF）外显子正在通过不等交换的过程进行协同进化。我们开发了一种基于长距离 PCR 的检测方法，以扩增大约 12kb 长的外显子，该外显子在串联阵列中包含可变数量的 303 或 306nt 长的重复。我们将此程序应用于 M. Wayne 和 L. Higgins 建立的黑腹果蝇突变积累系。在这些系中发现了 9 种新的重复长度变异体，使我们能够测量 PF 外显子中不等交换的速率。由于多种原因，该速率是低估的，为每代 7.05×10(-4)个交换。
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