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本文引用的文献
1
Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.
Nucleic Acids Res. 1988 Oct 25;16(20):9677-86. doi: 10.1093/nar/16.20.9677.
2
Restriction endonuclease cleavage at the termini of PCR products.
Biotechniques. 1990 Sep;9(3):304, 306.
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