A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors.
作者信息
Holton T A, Graham M W
机构信息
Calgene Pacific Pty. Ltd., Collingwood, Victoria, Australia.
出版信息
Nucleic Acids Res. 1991 Mar 11;19(5):1156. doi: 10.1093/nar/19.5.1156.
Abstract
摘要
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Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.原核生物和真核生物DNA聚合酶催化的新型非模板核苷酸添加反应。
Nucleic Acids Res. 1988 Oct 25;16(20):9677-86. doi: 10.1093/nar/16.20.9677.
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Restriction endonuclease cleavage at the termini of PCR products.在PCR产物末端进行限制性内切核酸酶切割。
Biotechniques. 1990 Sep;9(3):304, 306.
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