Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.
作者信息
Marchuk D, Drumm M, Saulino A, Collins F S
机构信息
Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 4810-0650.
出版信息
Nucleic Acids Res. 1991 Mar 11;19(5):1154. doi: 10.1093/nar/19.5.1154.
Abstract
摘要
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本文引用的文献
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Direct cloning and sequence analysis of enzymatically amplified genomic sequences.酶促扩增基因组序列的直接克隆与序列分析
Science. 1986 Sep 5;233(4768):1076-8. doi: 10.1126/science.3461561.
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Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.用于镰状细胞贫血诊断的β-珠蛋白基因组序列的酶促扩增及限制性酶切位点分析。
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A simple method for site-directed mutagenesis using the polymerase chain reaction.一种利用聚合酶链反应进行定点诱变的简单方法。
Nucleic Acids Res. 1989 Aug 25;17(16):6545-51. doi: 10.1093/nar/17.16.6545.
4
Using the polymerase chain reaction to modify expression plasmids for epitope mapping.利用聚合酶链反应修饰表达质粒用于表位作图。
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5
Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.原核生物和真核生物DNA聚合酶催化的新型非模板核苷酸添加反应。
Nucleic Acids Res. 1988 Oct 25;16(20):9677-86. doi: 10.1093/nar/16.20.9677.
6
Ligation-independent cloning of PCR products (LIC-PCR).聚合酶链式反应（PCR）产物的不依赖连接酶克隆（LIC-PCR）。
Nucleic Acids Res. 1990 Oct 25;18(20):6069-74. doi: 10.1093/nar/18.20.6069.
7
PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products.PCR诱导（无连接酶）亚克隆：一种快速可靠的亚克隆聚合酶链反应（PCR）产物的方法。
Nucleic Acids Res. 1990 Apr 11;18(7):1920. doi: 10.1093/nar/18.7.1920.
8
DNA mutagenesis and recombination.DNA诱变与重组
Nature. 1990 Apr 19;344(6268):793-4. doi: 10.1038/344793a0.
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Restriction endonuclease cleavage at the termini of PCR products.在PCR产物末端进行限制性内切核酸酶切割。
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