Universite de Strasbourg, France.
Phytopathology. 2010 Apr;100(4):384-9. doi: 10.1094/PHYTO-100-4-0384.
Grapevine fanleaf virus (GFLV) is vectored specifically from grapevine to grapevine by the ectoparasitic nematode Xiphinema index. Limited information is available on the vector competency of X. index populations from diverse geographical origins. We determined the transmissibility of two GFLV strains showing 4.6% amino acid divergence within their coat protein (e.g., strains F13 and GHu) by seven clonal lines of X. index developed from seven distinct populations from the Mediterranean basin (Cyprus, southern France, Israel, Italy, and Spain), northern France, and California. X. index lines derived from single adult females were produced on fig (Ficus carica) plants to obtain genetically homogenous aviruliferous clones. A comparative reproductive rate analysis on Vitis rupestris du Lot and V. vinifera cv. Cabernet Sauvignon showed significant differences among clones, with the single-female Cyprus line showing the highest rate (30-fold the initial population) and the Spain and California lines showing the lowest rate (10-fold increase), regardless of the grapevine genotype. However, there was no differential vector competency among the seven X. index lines for GFLV strains F13 and GHu. The implications of our findings for the dynamic of GFLV transmission in vineyards and screening of Vitis spp. for resistance to GFLV are discussed.
葡萄扇叶病毒（GFLV）由外寄生线虫 Xiphinema index 特异性地从葡萄传播到葡萄。关于来自不同地理起源的 X. index 种群的媒介能力，信息有限。我们通过来自地中海盆地（塞浦路斯、法国南部、以色列、意大利和西班牙）、法国北部和加利福尼亚的七个不同种群开发的七个克隆系，确定了两种 GFLV 株系（例如 F13 和 GHu 株系）的传染性，它们在外壳蛋白中存在 4.6%的氨基酸差异。X. index 线系是从单一成年雌性线虫在 fig（Ficus carica）植物上产生的，以获得遗传同质的无毒克隆系。对 Vitis rupestris du Lot 和 V. vinifera cv. Cabernet Sauvignon 的比较繁殖率分析表明，克隆系之间存在显著差异，塞浦路斯单一雌性线系的繁殖率最高（初始种群的 30 倍），西班牙和加利福尼亚线系的繁殖率最低（增加 10 倍），无论葡萄基因型如何。然而，对于 GFLV 株系 F13 和 GHu，七个 X. index 线系之间没有差异媒介能力。我们的研究结果对葡萄扇叶病毒在葡萄园传播的动态和筛选葡萄属对葡萄扇叶病毒的抗性具有重要意义。
