The David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
Int J Radiat Oncol Biol Phys. 2010 Mar 15;76(4):973-7. doi: 10.1016/j.ijrobp.2009.11.038.
To image a genetically engineered mouse model of non-small-cell lung cancer with micro-computed tomography (micro-CT) to measure tumor response to radiation therapy.
The Cre-loxP system was used to generate primary lung cancers in mice with mutation in K-ras alone or in combination with p53 mutation. Mice were serially imaged by micro-CT, and tumor volumes were determined. A comparison of tumor volume by micro-CT and tumor histology was performed. Tumor response to radiation therapy (15.5 Gy) was assessed with micro-CT.
The tumor volume measured with free-breathing micro-CT scans was greater than the volume calculated by histology. Nevertheless, this imaging approach demonstrated that lung cancers with mutant p53 grew more rapidly than lung tumors with wild-type p53 and also showed that radiation therapy increased the doubling time of p53 mutant lung cancers fivefold.
Micro-CT is an effective tool to noninvasively measure the growth of primary lung cancers in genetically engineered mice and assess tumor response to radiation therapy. This imaging approach will be useful to study the radiation biology of lung cancer.
利用微计算机断层扫描（micro-CT）对非小细胞肺癌的基因工程小鼠模型进行成像，以测量肿瘤对放射治疗的反应。
使用 Cre-loxP 系统在单独或同时携带 K-ras 突变和 p53 突变的小鼠中生成原发性肺癌。通过 micro-CT 对小鼠进行连续成像，并确定肿瘤体积。对 micro-CT 和肿瘤组织学测量的肿瘤体积进行了比较。使用 micro-CT 评估了放射治疗（15.5 Gy）对肿瘤的反应。
自由呼吸 micro-CT 扫描测量的肿瘤体积大于组织学计算的体积。尽管如此，这种成像方法表明，携带突变型 p53 的肺癌比野生型 p53 的肺肿瘤生长得更快，并且还表明放射治疗使 p53 突变型肺癌的倍增时间增加了五倍。
micro-CT 是一种非侵入性测量基因工程小鼠中原发性肺癌生长并评估肿瘤对放射治疗反应的有效工具。这种成像方法将有助于研究肺癌的放射生物学。
