一种在风湿性心脏病大鼠模型中诱导自身免疫性心瓣膜炎的替代技术。
An alternative technique for the induction of autoimmune valvulitis in a rat model of rheumatic heart disease.
机构信息
Microbiology and Immunology, School of Veterinary and Biomedical Sciences, James Cook University, Townsville 4811, Australia.
出版信息
J Immunol Methods. 2010 Apr 15;355(1-2):80-5. doi: 10.1016/j.jim.2010.02.013. Epub 2010 Mar 3.
We currently use a rat model in our investigations into human rheumatic heart disease (RHD). This model traditionally involves footpad immunization with antigen emulsified in complete Freund's adjuvant (CFA). Trials to find an alternative adjuvant to CFA which produced a Th1 type response in the rats resulting in carditis were unsuccessful. However, hock immunization was found to produce the desired valvular pathology without the adverse inflammatory side-effects associated with CFA. We therefore consider the hock an ideal site for immunization, particularly when using CFA.
我们目前在研究人类风湿性心脏病（RHD）时使用大鼠模型。该模型传统上涉及用完全弗氏佐剂（CFA）乳化的抗原进行足底免疫。寻找可替代 CFA 的佐剂的试验未能成功，因为这些佐剂在大鼠中产生 Th1 型反应导致心炎。然而，发现后踝关节免疫可产生所需的瓣膜病理学，而不会产生与 CFA 相关的不良炎症副作用。因此，我们认为后踝关节是免疫接种的理想部位，特别是在使用 CFA 时。
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