Abdel-Aziem Sekena H, El-Nekeety Aziza A, Barakat Ibrahim A, Mohamed Mohamed I, Abdel-Wahhab Mosaad A
Cell Biology Department, National Research Center, Dokki, Cairo, Egypt.
Exp Toxicol Pathol. 2011 May;63(4):337-44. doi: 10.1016/j.etp.2010.02.008. Epub 2010 Mar 4.
Cadmium (Cd) is a non-essential element and is a widespread environmental pollutant. Exposure to cadmium can result in cytotoxic, carcinogenic and mutagenic effects. The aim of the current work was to evaluate the protective effect of Aquilegia vulgaris extract against the oxidative stress and the genotoxicity induced by Cd using the chromosomal aberrations in somatic and germ cells assay and random amplified polymorphism DNA (RAPD-PCR) analysis. Forty male Balb/c mice were divided into four groups including the control group, Cd-treated group and the groups treated with the extract alone or plus Cd. The results indicated that Cd increased serum ALT, AST, urea, LDH, CK, lipid peroxidation in liver tissue accompanied with a significant decrease in GPX and SOD. Cd also increased the number of chromosomal aberrations in bone marrow and spermatocytes including structural and numerical aberrations. Animals treated with the extract alone were comparable to the control regarding all the tested parameters. The extract succeeded in preventing or diminishing the oxidative stress and the clastogenic effects of Cd. It could be concluded that Aquilegia vulgaris extract is a promising protective agent against oxidative stress and genotoxicity during the exposure to Cd.
镉（Cd）是一种非必需元素，是一种广泛存在的环境污染物。接触镉会导致细胞毒性、致癌和致突变作用。当前工作的目的是使用体细胞和生殖细胞中的染色体畸变分析以及随机扩增多态性DNA（RAPD-PCR）分析，评估普通耧斗菜提取物对镉诱导的氧化应激和遗传毒性的保护作用。将40只雄性Balb/c小鼠分为四组，包括对照组、镉处理组以及单独用提取物处理或提取物加镉处理的组。结果表明，镉会增加血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、尿素、乳酸脱氢酶（LDH）、肌酸激酶（CK）以及肝组织中的脂质过氧化水平，同时伴随着谷胱甘肽过氧化物酶（GPX）和超氧化物歧化酶（SOD）的显著降低。镉还会增加骨髓和精母细胞中的染色体畸变数量，包括结构和数目畸变。单独用提取物处理的动物在所有测试参数方面与对照组相当。提取物成功预防或减轻了镉的氧化应激和致断裂作用。可以得出结论，普通耧斗菜提取物是一种在接触镉期间对抗氧化应激和遗传毒性的有前景的保护剂。
