Cell Biology Department, National Research Center, Dokki, Cairo, Egypt.
Toxicon. 2010 Aug 1;56(1):8-18. doi: 10.1016/j.toxicon.2010.03.006. Epub 2010 Mar 15.
Exposure to fumonisins (FB) is known to have toxic and carcinogenic effects in different animal species, and to express toxicity in cells via the induction of oxidative stress. The aim of the current study was to evaluate the protective effects of the ethanol extract of Aquilegia vulgaris L. against the oxidative stress and the genotoxicity using micronucleus assay and random amplified polymorphism DNA (RAPD-PCR) in FB-treated rats. Sixty mature female Sprague-Dawley were divided into six treatment groups and treated for 4 weeks as follow: the control group, the group fed FB-contaminated diet (200 mg/kg diet), the groups treated orally with the extract (5 and 10 mg/kg bw) and the groups fed FB-contaminated diet and treated with the extract at the two doses. The results showed that treatment with FB alone disturbed lipid profile in serum, increases Sa/So ratio, induces micronucleated polychromatic erythrocytes (Mn-PCEs) in bone marrow, increases DNA and RNA in liver accompanied with significant changes in histological picture The extract alone at the two tested doses did not induce any significant changes in the biochemical or histological picture. The combined treatment showed significant improvements in all biochemical, cytogenetic parameters tested and histological pictures in the liver tissues. Moreover, this improvement was more pronounced in the group received the high dose of the extract. It could be concluded that the ethanol extract of A. vulgaris induced its protective effect via the increase in the antioxidant capacity, inhibition of lipid peroxidation and scavenging of free radicals.
暴露于伏马菌素（FB）已知在不同动物物种中具有毒性和致癌作用，并通过诱导氧化应激在细胞中表达毒性。本研究旨在评估毛茛乙醇提取物对 FB 处理大鼠氧化应激和遗传毒性的保护作用，采用微核试验和随机扩增多态性 DNA（RAPD-PCR）进行评估。将 60 只成熟雌性 Sprague-Dawley 大鼠分为 6 个治疗组，连续 4 周进行处理：对照组、FB 污染饮食组（200mg/kg 饮食）、提取物（5 和 10mg/kg bw）口服处理组和 FB 污染饮食+提取物（两种剂量）处理组。结果表明，单独用 FB 处理会扰乱血清中的脂质谱，增加 Sa/So 比值，诱导骨髓中多色性红细胞的微核形成（Mn-PCEs），增加肝脏中的 DNA 和 RNA，同时肝脏组织的组织学图片也发生了显著变化。单独使用两种测试剂量的提取物不会引起生化或组织学图片的任何显著变化。联合治疗在所有测试的生化、细胞遗传学参数和肝脏组织学图片上均显示出显著改善。此外，高剂量提取物组的改善更为明显。可以得出结论，毛茛乙醇提取物通过增加抗氧化能力、抑制脂质过氧化和清除自由基来诱导其保护作用。
