Department of Pharmacy, Xijing Hospital, The Fourth Military Medical University, No. 127, Changle West Road, Xi'an, China.
J Ethnopharmacol. 2010 May 4;129(1):1-4. doi: 10.1016/j.jep.2010.02.023. Epub 2010 Mar 4.
Safflower is a popular Traditional Chinese Medicine (TCM) to invigorate the blood and dispel 'blood stasis', which arises from poor blood circulation. The differences of pharmacokinetic properties between normal and blood stasis syndrome rats were seldom reported.
The present study was conducted to evaluate the pharmacokinetics of hydroxysafflower yellow A (HSYA) following oral administration of hydroxysafflower yellow A and safflower extract with approximately the same dose of HSYA 100mg/kg in both normal and acute blood stasis rats.
The animals were orally administered with HYSA monomer and safflower extract. The blood samples were collected according to the time schedule. The concentrations of HSYA in rat plasma were determined by HPLC. Various pharmacokinetic parameters were estimated from the plasma concentration versus time data using non-compartmental methods.
It was found that AUC(0-t), C(max), Vd and CL of HSYA in both HSYA monomer and safflower extract in acute blood stasis rats were with significant difference (P<0.05) comparing with that in normal rats.
The results indicated that HSYA was with high uptake and eliminated slowly in the animals with blood stasis syndrome, suggesting that the rate and extent of drug metabolism was altered in acute blood stasis animals.
红花是一种常用的传统中药，具有活血化瘀的功效，可改善血液循环不畅导致的“血瘀”症状。然而，关于正常大鼠和血瘀证大鼠之间药代动力学性质差异的研究却鲜有报道。
本研究旨在评估羟红花黄色素 A（HSYA）在正常和急性血瘀证大鼠中的药代动力学特征。红花提取物和 HSYA 单体经口给予大鼠，剂量均约为 HSYA100mg/kg。
动物经口给予 HSYA 单体和红花提取物。根据时间安排采集血样。采用 HPLC 法测定大鼠血浆中 HSYA 的浓度。采用非房室模型法，根据血药浓度-时间数据估算各种药代动力学参数。
与正常大鼠相比，急性血瘀证大鼠给予 HSYA 单体和红花提取物后，HSYA 的 AUC(0-t)、C(max)、Vd 和 CL 均有显著差异（P<0.05）。
结果表明，血瘀证动物对 HSYA 的摄取增加，消除缓慢，提示血瘀证动物的药物代谢速率和程度发生了改变。
