Zhou Qixiu, He Mei, Jin Qiong, Gao Shijia, Yang Zhuya, Zhu Peifeng, Tan Wenhong, Liu Lu
Yunnan Yunzhong Institute of Nutrition and health, College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, China.
Front Med (Lausanne). 2024 Apr 30;11:1343179. doi: 10.3389/fmed.2024.1343179. eCollection 2024.
As one of the most common gynecological disorders, PD significantly impacts the quality of life for women. TSD, a well-known traditional Chinese medical prescription, has gained popularity for its use in treating gynecological cold coagulation and blood stasis syndromes such as PD. However, the lack of comprehensive data hinders our understanding of its molecular mechanism.
The objective of the present study is to investigate the therapeutic effects of TSD on PD and elucidate its plausible mechanism.
HPLC was employed to confirm the presence of the principal metabolites of TSD. The rat model of PD was induced by OT exposure following IWM and EB pretreatment, and subsequently treated with TSD via gastric gavage. The effects and potential mechanisms of TSD on PD rats were explored, encompassing general behavior, morphological alterations in the uterus and ovaries, biochemical indicators in the uterus and serum, and levels of proteins related to the PI3K/AKT signaling pathway.
Gallic acid, hydroxysafflower yellow A, albiflorin, paeoniflorin, and ferulic acid were determined to be the primary active metabolites of TSD. The pharmacological studies yielded results indicating the successful establishment of the PD model in rats. Additionally, TSD demonstrated its ability to protect PD rats by ameliorating general behavior, mitigating pathological damage to uterine and ovarian tissues, and modulating the expression levels of correlated factors (PGE2, PGF2, Ca2+, TXB2, IL-6, TNF-, NO, and COX-2) as well as p-PI3K/PI3K and p-AKT/AKT proteins.
TSD exhibited protective effects against PD in rats through its interaction with multiple targets including P13K/AKT signaling pathway, indicating that TSD holds therapeutic potential for PD treatment and providing evidence supporting the rational utilization of TSD.
痛经（PD）作为最常见的妇科疾病之一，严重影响女性的生活质量。温经汤（TSD）是一种著名的中药方剂，因其在治疗妇科寒凝血瘀证如痛经方面的应用而受到欢迎。然而，缺乏全面的数据阻碍了我们对其分子机制的理解。
本研究的目的是探讨温经汤对痛经的治疗作用并阐明其可能的机制。
采用高效液相色谱法（HPLC）确认温经汤主要代谢产物的存在。通过宫内粘连模型（IWM）和乙烯雌酚（EB）预处理后暴露于氧化应激（OT）诱导大鼠痛经模型，随后通过灌胃给予温经汤进行治疗。探讨温经汤对痛经大鼠的作用及潜在机制，包括一般行为、子宫和卵巢的形态学改变、子宫和血清中的生化指标以及与磷脂酰肌醇-3激酶/蛋白激酶B（PI3K/AKT）信号通路相关的蛋白质水平。
确定没食子酸、羟基红花黄色素A、白花芍药苷、芍药苷和阿魏酸为温经汤的主要活性代谢产物。药理学研究结果表明成功建立了大鼠痛经模型。此外，温经汤通过改善一般行为、减轻子宫和卵巢组织的病理损伤以及调节相关因子（前列腺素E2、前列腺素F2、钙离子、血栓素B2、白细胞介素-6、肿瘤坏死因子-α、一氧化氮和环氧化酶-2）以及磷酸化PI3K/PI3K和磷酸化AKT/AKT蛋白的表达水平，显示出对痛经大鼠的保护能力。
温经汤通过与包括PI3K/AKT信号通路在内的多个靶点相互作用，对大鼠痛经表现出保护作用，表明温经汤在痛经治疗中具有治疗潜力，并为温经汤的合理应用提供了证据支持。
