Setor de Entomologia Médica e Forense, Instituto Oswaldo Cruz/IOC/FIOCRUZ, Pavilhão Carlos Chagas, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil.
Micron. 2010 Jul;41(5):518-25. doi: 10.1016/j.micron.2010.01.008. Epub 2010 Feb 11.
Morphological studies in male reproductive system of insect, and accessory glands functions, can contribute to the knowledge of reproductive aspects in Triatominae. The present study aims to describe the structure of male reproductive system under scanning electron microscopy, and the ultrastructure of accessory glands during the process of maturation, in T. brasiliensis and T. melanica of different ages. These system consist of two testis, two vas deferens, two seminal vesicles, four pair of accessory glands and one ejaculatory duct. The four mesodermal male accessory glands of T. melanica and T. brasiliensis, present secretion storage in the first days of adult life in starved males. During the maturation process of male accessory glands of T. brasiliensis and T. melanica, granules with different sizes, shapes and electron density were seen. In T. melanica small vacuoles are released into the gland lumen in one-day-old males, however, after three and five days few granules are found in the cell cytoplasm. The secretory granules in T. brasiliensis increase in size and amount, in the five days old adult. The secretory pathway in the male accessory glands of the two species seems to be merocrine and apocrine suggesting that different substances are being produced at different times. The cell microvilli in T. brasiliensis are longer and narrower, these can be absent in some regions of the cell surface of T. melanica. Maturation of male accessory glands in the triatomine species studied occurred without adult blood meal. Its function can led to new insights for the comprehension of reproductive aspects in Triatominae.
昆虫雄性生殖系统和附腺功能的形态学研究，可以为了解锥蝽的生殖方面提供知识。本研究旨在通过扫描电子显微镜描述雄性生殖系统的结构，以及在不同年龄的 T. brasiliensis 和 T. melanica 中附腺在成熟过程中的超微结构。该系统由两个睾丸、两条输精管、两个精囊、四个附腺对和一个射精管组成。T. melanica 和 T. brasiliensis 的四个中胚层雄性附腺，在饥饿雄性的成年初期就有分泌物储存。在 T. brasiliensis 和 T. melanica 雄性附腺的成熟过程中，观察到大小、形状和电子密度不同的颗粒。在 1 日龄雄性中，小泡被释放到 T. melanica 的腺腔中，然而，在 3 日龄和 5 日龄时，细胞细胞质中发现的颗粒很少。在 5 日龄成年期，T. brasiliensis 的分泌颗粒体积和数量增加。这两种物质的产生似乎是不同的。两种物种的雄性附腺的分泌途径似乎是顶浆分泌和胞吐，这表明在不同的时间产生不同的物质。T. brasiliensis 的细胞微绒毛又长又窄，而 T. melanica 的细胞表面的一些区域可能没有这些微绒毛。研究中三种锥蝽的雄性附腺成熟发生在没有成虫血餐的情况下。它的功能可以为理解锥蝽的生殖方面提供新的见解。
