taxonomical 分类学的，分类学上的；integrative 综合的；phylogenetic 系统发生的；identification 鉴定，识别；Triatoma rubrofasciata 棕褐异锥蝽；recorded 有记录的；Zhangzhou 漳州市；Fujian Province 福建省；Maoming 茂名市；Guangdong Province 广东省；China 中国 中国福建省漳州市和广东省茂名市首次记录的棕褐异锥蝽的分类学综合与系统发生鉴定。
Taxonomic integrative and phylogenetic identification of the first recorded Triatoma rubrofasciata in Zhangzhou, Fujian Province and Maoming, Guangdong Province, China.
机构信息
Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, China.
出版信息
Infect Dis Poverty. 2019 Aug 13;8(1):70. doi: 10.1186/s40249-019-0579-8.
BACKGROUND
Most species of Triatominae live exclusively in Latin America. However, one species, Triatoma rubrofasciata, has been recorded in the Americas as well as in various port areas in Africa and Asia. An increasing number of T. rubrofasciata have been reported in southern China in recent years. However, the origin of this invasive insect vector in China remains unknown, therefore, accurate identification and phylogenetic analysis of the bugs are urgently needed.
METHODS
A total of seven triatomine insect specimens were found and collected from Maoming City, Guangdong Province, China (GDMM) and Zhangzhou City, Fujian Province, China (FJZZ), respectively. The obtained insect vector specimens were observed under a dissecting microscope for morphological classification and then the genomic DNA was extracted, and the 16S ribosomal RNA (rRNA), 28S rRNA as well as cytochrome oxidase subunit I (COI) genes of the species were amplified and sequenced. Subsequently, molecular phylogenetic analyses based on multiple alignments of the above genes were conducted in order to identify the species and determine the phylogenetic origin approximation accurately.
RESULTS
The triatomine insects collected from GDMM and FJZZ were identified as Triatoma rubrofasciata using morphological and genetic analyses. All of the Chinese T. rubrofasciata captured in FJZZ, GDMM and other localities in southern China, together with a Vietnamese and Brazilian strain, formed a new, cohesive clade. T. rubrofasciata in GDMM and FJZZ are likely derived from strains found in Vietnam or Brazil.
CONCLUSIONS
To the best of our knowledge, this is the first record of the invasive insect T. rubrofasciata, which is likely derived from strains native to Vietnam or Brazil, in both Maoming City, Guangdong Province and Zhangzhou City, Fujian Province of China. A comparison of the DNA sequences of the 16 s rRNA, 28 s rRNA and COI genes confirmed the specific identification of T. rubrofasciata, and its potential origin in China is based on the phylogenetic analyses undertaken in this study. More targeted interventions and improved entomological surveillance are urgently needed to control the spread of this haematophagous insect in China.
背景
大多数三锥虫属物种仅生活在拉丁美洲。然而，有一种物种，红带三锥虫，已在美洲以及非洲和亚洲的各个港口地区被记录。近年来，中国南方报告的红带三锥虫数量不断增加。然而，这种入侵性昆虫媒介在中国的起源仍然未知，因此，急需对这些虫子进行准确的鉴定和系统发育分析。
方法
从中国广东省茂名市（GDMM）和福建省漳州市（FJZZ）分别采集并发现了总共 7 只三锥虫昆虫标本。在解剖显微镜下观察所获得的昆虫载体标本，进行形态分类，然后提取基因组 DNA，扩增并测序其 16S 核糖体 RNA（rRNA）、28S rRNA 以及细胞色素氧化酶亚基 I（COI）基因。随后，基于上述基因的多重比对进行分子系统发育分析，以准确鉴定物种并确定其近似的系统发育起源。
结果
通过形态学和遗传学分析，确定从 GDMM 和 FJZZ 采集的三锥虫昆虫为红带三锥虫。在中国 FJZZ、GDMM 和中国南方其他地区捕获的所有中国红带三锥虫，以及越南和巴西的一个株系，形成了一个新的、有凝聚力的分支。GDMM 和 FJZZ 的红带三锥虫可能源自越南或巴西的株系。
结论
据我们所知，这是在广东省茂名市和福建省漳州市首次记录到可能源自越南或巴西本土株系的入侵性昆虫红带三锥虫。对 16s rRNA、28s rRNA 和 COI 基因的 DNA 序列进行比较，确认了红带三锥虫的特异性鉴定，并且根据本研究进行的系统发育分析，确定了其在中国的潜在起源。迫切需要更有针对性的干预措施和改进的昆虫学监测，以控制这种吸血昆虫在中国的传播。
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