Chaud M A, Gonzalez E T, Franchi A M, Fernandez Pardal J, Gimeno A L, Gimeno M A
Centro de Estudios Farmácológicos y Botánicos (CEFYBO), Universidad de Buenos Aires, Argentina.
Prostaglandins Leukot Essent Fatty Acids. 1991 Feb;42(2):119-23. doi: 10.1016/0952-3278(91)90078-j.
The spontaneous isometric developed tension (IDT), the synthesis and release of prostaglandins (PGs) into the incubating medium and the metabolism of triglycerides (TGs) in uterine strips isolated from controls and chronic ethanol fed rats, were studied. In order to observe how the uterus of rats fed alcohol reacts during a situation of metabolic emergency, the above mentioned studies were done in the presence or in the absence of glucose in the incubating medium. The decrement of IDT as time progressed was significantly greater in strips obtained from rats which had been drinking 20% ETOH than in controls. Nevertheless, the absolute magnitude of the initial IDT was similar in both groups. On the other hand, the decline of the frequency of contractions (FC) of uterine strips isolated from controls and from ETOH-exposed rats, after 60 min of spontaneous activity was similar. When the uterine strips isolated from ETOH-exposed and from control rats were suspended in glucose-free solution they exhibited the same decrement of IDT and FC after 60 min of activity. The basal release of PGE1 and PGE2 was similar in control tissues incubated in medium containing glucose, but the output of PGE2 was significantly smaller than that of PGE1 in uterine strips isolated from ETOH-exposed rats. The production of PGE1 and PGE2 by uteri suspended in glucose-free medium was similar in control preparations. On the contrary the release of both PGs differs in uterine strips from ETOH-exposed rats, i.e. the output of PGE2 was significantly smaller than in controls and the release of PGE1 increased around 4-fold in comparison with controls.(ABSTRACT TRUNCATED AT 250 WORDS)
研究了从对照组和长期乙醇喂养大鼠分离的子宫条带的自发等长收缩张力（IDT）、前列腺素（PGs）向孵育培养基中的合成与释放以及甘油三酯（TGs）的代谢。为了观察饮酒大鼠的子宫在代谢紧急情况下的反应，上述研究在孵育培养基中存在或不存在葡萄糖的情况下进行。随着时间的推移，饮用20%乙醇的大鼠子宫条带的IDT下降幅度明显大于对照组。然而，两组初始IDT的绝对值相似。另一方面，对照组和乙醇暴露大鼠分离的子宫条带在自发活动60分钟后，收缩频率（FC）的下降相似。当将乙醇暴露大鼠和对照大鼠分离的子宫条带悬浮在无葡萄糖溶液中时，活动60分钟后它们的IDT和FC下降相同。在含葡萄糖培养基中孵育的对照组织中，PGE1和PGE2的基础释放相似，但在乙醇暴露大鼠分离的子宫条带中，PGE2的产量明显低于PGE1。悬浮在无葡萄糖培养基中的子宫产生PGE1和PGE2在对照制剂中相似。相反，乙醇暴露大鼠子宫条带中两种PGs的释放不同，即PGE2的产量明显低于对照组，PGE1的释放量比对照组增加了约4倍。（摘要截短至250字）
