Jawerbaum A, Roselló Catafau J, Gonzalez E T, Novaro V, Gomez G, Gelpi E, Gimeno A L, Gimeno M A
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET), Buenos Aires, Argentina.
Prostaglandins. 1994 Feb;47(2):81-96. doi: 10.1016/0090-6980(94)90079-5.
Spontaneous contractile activity, glucose (Glu), glycogen (GLY), triglyceride (TG) metabolism and eicosanoid production, was evaluated in isolated uterine strips from control and non-insulin-dependent diabetic rats on day 10 of pregnancy. Metabolism of Glu, levels of GLY and TG and eicosanoid production were also studied in day 10 embryos obtained from both experimental groups. "In vitro" isometric developed tension (IDT), was similar at 0 hr in control and diabetic uterine preparations, but IDT was decreased after a 60 min incubation in the diabetic group. The frequency of contractions (FC) was similar at 0 hr and after 60 min incubation in both experimental groups. On the other hand, the production of 14CO2 from U14C-glucose was lower in isolated uteri and embryos obtained from diabetic rats than in controls. Initial TG levels were similar in uteri isolated from control and diabetic rats, and higher in embryos obtained from diabetic mothers than in controls. Levels of TG in uterine strips suspended in Glu or Glu-free medium did not differ at 0 hr or at 60 min either in controls or in diabetic rats. On the contrary GLY levels in uterine strips from diabetic animals were higher than in controls, whereas in embryos from diabetic mothers GLY levels were similar to controls. Levels of GLY in uterine strips from controls and diabetic animals decreased after 60 min incubation only in the absence of Glu in the incubation medium. Production of PGE2, PGE1, 6-keto-PGF1 alpha, PGF2 alpha, TXB2 and LTB4 was studied in uterine strips and embryos obtained from control and diabetic rats. No differences were found between control and diabetic uterine prostanoid production, but lower production of LTB4 was observed in diabetic uteri. However production of PGE2 and PGF2 alpha was greater in embryos obtained from diabetic mothers than in controls. In this study, we observed lower uterine metabolic alterations than in the pancreatectomized diabetic rat model studied previously, but important anomalies in the embryos obtained from non-insulin-dependent diabetic mother were found.
在妊娠第10天，对来自对照大鼠和非胰岛素依赖型糖尿病大鼠的离体子宫条的自发收缩活动、葡萄糖（Glu）、糖原（GLY）、甘油三酯（TG）代谢及类花生酸生成进行了评估。还对来自两个实验组的妊娠第10天胚胎的Glu代谢、GLY和TG水平及类花生酸生成进行了研究。“体外”等长收缩张力（IDT）在0小时时，对照和糖尿病子宫标本相似，但糖尿病组在孵育60分钟后IDT降低。两个实验组在0小时和孵育60分钟后的收缩频率（FC）相似。另一方面，从糖尿病大鼠获得的离体子宫和胚胎中，U14C -葡萄糖产生的14CO2比对照组低。从对照大鼠和糖尿病大鼠分离的子宫中初始TG水平相似，而从糖尿病母亲获得的胚胎中的TG水平高于对照组。在0小时或60分钟时，无论是对照组还是糖尿病大鼠，悬浮于含Glu或不含Glu培养基中的子宫条中的TG水平均无差异。相反，糖尿病动物子宫条中的GLY水平高于对照组，而糖尿病母亲胚胎中的GLY水平与对照组相似。仅在孵育培养基中不含Glu时，对照组和糖尿病动物子宫条中的GLY水平在孵育60分钟后降低。对从对照和糖尿病大鼠获得的子宫条和胚胎中的前列腺素E2（PGE2）,前列腺素E1（PGE1）, 6 -酮 -前列腺素F1α（6 - keto - PGF1α）,前列腺素F2α（PGF2α）,血栓素B（TXB2）和白三烯B4（LTB4）的生成进行了研究。对照和糖尿病子宫前列腺素生成之间未发现差异，但糖尿病子宫中LTB4的生成较低。然而，从糖尿病母亲获得的胚胎中PGE2和PGF2α的生成比对照组更高。在本研究中，我们观察到子宫代谢改变比先前研究的胰腺切除糖尿病大鼠模型中的要低，但发现从非胰岛素依赖型糖尿病母亲获得的胚胎存在重要异常。
