Gonzales E T, Franchi A M, Jawerbaum A, Aliberti S, Gimeno A L, Gimeno M A
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Cientificas y Tecnicas de la República Argentina (CONICET), Buenos Aires.
Prostaglandins Leukot Essent Fatty Acids. 1993 Jul;49(1):495-501. doi: 10.1016/0952-3278(93)90037-w.
The constancy of spontaneous isometric developed tension (IDT) and the metabolism of triglycerides (TGs), U-14C-Glucose and 14C-arachidonic acid (14C-AA) in uterine strips isolated from controls and from chronic ethanol (ETOH) fed pregnant rats were explored. The studies were performed on isolated uterine strips suspended in glucose-containing as well as a glucose-free medium. The spontaneous decrement of IDT as time progressed after tissue isolation and mounting was significantly higher in tissue preparations obtained from pregnant rats drinking 20% ETOH, than the controls. This situation was evident in uterine strips isolated from rats at 10 and at 16 days of pregnancy, both in solutions containing glucose or in glucose-free conditions. On the other hand, uterine strips isolated from control rats at 7, 10 and 16 days of pregnancy exhibited almost no decrement of IDT after 60 min of activity in a solution containing glucose or in a glucose-free medium. The absolute values of TGs in uteri obtained from rats drinking ETOH were significantly greater (p < 0.001) than in non-drinking controls. TG levels did not differ at 0 min (initial or postisolation) to those at 60 min in control uterine preparations obtained from pregnant rats at 7, 10 or 16 days of pregnancy and incubated either in a medium with or without glucose. On the contrary, in strips from ETOH-fed animals isolated on the same day of pregnancy, TG levels determined at 60 min following isolation and mounting were significantly lower, when glucose was present or absent from the suspending solution.(ABSTRACT TRUNCATED AT 250 WORDS)
研究了从对照组和慢性乙醇（ETOH）喂养的怀孕大鼠分离出的子宫条带中自发等长收缩张力（IDT）以及甘油三酯（TGs）、U-14C-葡萄糖和14C-花生四烯酸（14C-AA）的代谢情况。实验在悬浮于含葡萄糖以及无糖培养基中的分离子宫条带上进行。从饮用20% ETOH的怀孕大鼠获得的组织标本中，组织分离和固定后随着时间推移IDT的自发下降显著高于对照组。这种情况在怀孕10天和16天的大鼠分离出的子宫条带中很明显，无论在含葡萄糖溶液还是无糖条件下均如此。另一方面，从怀孕7天、10天和16天的对照大鼠分离出的子宫条带，在含葡萄糖或无糖培养基中活动60分钟后，IDT几乎没有下降。饮用ETOH的大鼠子宫中TG的绝对值显著高于（p < 0.001）非饮用对照组。怀孕7天、10天或16天的大鼠的对照子宫标本，在含或不含葡萄糖的培养基中孵育，0分钟（初始或分离后）时的TG水平与60分钟时无差异。相反，在怀孕同一天分离的ETOH喂养动物的条带中，无论悬浮溶液中有无葡萄糖，分离和固定后60分钟测定的TG水平均显著降低。（摘要截断于250字）
