Department of Science Education, Jeju National University, Jeju 690-756, Republic of Korea.
Int J Syst Evol Microbiol. 2011 Feb;61(Pt 2):264-270. doi: 10.1099/ijs.0.020313-0. Epub 2010 Mar 5.
A novel actinomycete, designated strain Sco-B14(T), was isolated from volcanic ash collected near Darangshi Oreum (a parasitic or satellite volcano) in Jeju, Republic of Korea. The organism formed well-developed, branched substrate mycelium, on which short chains of non-motile spores were arranged singly or in clusters. Aerial mycelium was not produced. Globose bodies were observed. The reverse colour of colonies was light brown to brown. Diffusible pigments were produced on ISP medium 3 and oatmeal-nitrate agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Sco-B14(T) formed a lineage within the family Micromonosporaceae and was distinct from established genera. The 16S rRNA gene sequence similarity of strain Sco-B14(T) to members of related genera of the family was 95.0-95.7 % to type strains of Catellatospora species, 94.7 % to Hamadaea tsunoensis IMSNU 22005(T), 94.7 % to Longispora albida K97-0003(T) and 94.0 % to Catelliglobosispora koreensis LM 042(T). 3-Hydroxydiaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. Whole-cell sugars were glucose, rhamnose, ribose, xylose, arabinose, galactose and mannose. The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The menaquinone profile contained MK-10(H(4)) (49 %), MK-9(H(4)) (24 %), MK-10(H(6)) (18 %) and MK-9(H(6)) (9 %). The predominant fatty acids were iso-C(15 : 0) and C(17 : 0). The DNA G+C content was 70.1 mol%. The combination of chemotaxonomic and phylogenetic data clearly separated the isolate from the type strains of all genera in the family Micromonosporaceae. On the basis of the phylogenetic and chemotaxonomic data presented in this paper, strain Sco-B14(T) is considered to represent a novel species of a new genus in the family Micromonosporaceae, for which the name Allocatelliglobosispora scoriae gen. nov., sp. nov. is proposed. The type strain of Allocatelliglobosispora scoriae is Sco-B14(T) (=KCTC 19661(T) =DSM 45362(T)).
一株新的放线菌，命名为 Sco-B14(T) 菌株，是从韩国济州岛 Darangshi Oreum（寄生或卫星火山）采集的火山灰中分离得到的。该菌形成发育良好的分枝基质菌丝，其上排列着单链或链状的非运动孢子。气生菌丝不发达。观察到球形小体。菌落的反面颜色为浅棕色到棕色。ISP 培养基 3 和燕麦粉硝酸盐琼脂上产生可扩散色素。基于 16S rRNA 基因序列的系统发育分析表明，菌株 Sco-B14(T) 在 Micromonosporaceae 科的家族内形成一个谱系，与已建立的属明显不同。菌株 Sco-B14(T)与相关属的成员的 16S rRNA 基因序列相似性为 95.0-95.7%到 Catellatospora 种的模式菌株，94.7%到 Hamadaea tsunoensis IMSNU 22005(T)，94.7%到 Longispora albida K97-0003(T)和 94.0%到 Catelliglobosispora koreensis LM 042(T)。3-羟基二氨基庚二酸是细胞壁肽聚糖中的诊断二氨基庚二酸。全细胞糖为葡萄糖、鼠李糖、核糖、木糖、阿拉伯糖、半乳糖和甘露糖。极性脂包括双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰肌醇。类异戊二烯醌图谱包含 MK-10(H(4))(49%)、MK-9(H(4))(24%)、MK-10(H(6))(18%)和 MK-9(H(6))(9%)。优势脂肪酸为 iso-C(15:0)和 C(17:0)。DNA G+C 含量为 70.1 mol%。化学分类和系统发育数据的结合清楚地将该分离株与 Micromonosporaceae 科中所有属的模式菌株区分开来。基于本文呈现的系统发育和化学分类数据，菌株 Sco-B14(T) 被认为代表了 Micromonosporaceae 科中新属的一个新种，建议将其命名为Allocatelliglobosispora scoriae 属。Allocatelliglobosispora scoriae 的模式菌株为 Sco-B14(T) (=KCTC 19661(T) =DSM 45362(T))。
