Sun Ye, Jiang Zhu-Ming, Zhao Li-Li, Su Jing, Yu Li-Yan, Tian Yong-Qiang, Zhang Yu-Qin
1Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
2Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education and College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, PR China.
Int J Syst Evol Microbiol. 2019 Jan;69(1):109-115. doi: 10.1099/ijsem.0.003114. Epub 2018 Nov 16.
The taxonomic position of an actinobacterium, designated CPCC 204380, which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China, was established using a polyphasic approach. Vegetative hyphae developed well and globose bodies formed from aged hyphae. Spore chains that differentiated from the vegetative hyphae contained non-motile rod-shaped spores. The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids. The acyl type of the peptidoglycan was glycolyl. Glucose, mannose, ribose and xylose were detected in whole-cell hydrolysates. The predominant menaquinone was MK-10(H8), followed by MK-10(H6) and MK-10(H4). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c. The genomic G+C content was 64.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204380 should be placed in the family Micromonosporaceae, in which it formed a distinct lineage next to the genera Rhizocola, Catellatospora, Catelliglobosispora, Hamadaea and Allocatelliglobosispora. It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602 (96.1 %), Catellatospora chokoriensis 2-25/1 (95.9 %), Catelliglobosispora koreensis DSM 44566 (95.9 %), Hamadaea tsunoensis DSM 44101 (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 (94.2 %), and less than 94.0 % sequence similarity with other validly described species. The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380 as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp. nov. is proposed. CPCC 204380 (=DSM 102292=KCTC 39746 ) is the type strain of the type species.
采用多相分类法确定了从中国新疆采集的沙拐枣植物根际土壤样品中分离出的一株放线菌（命名为CPCC 204380）的分类地位。该菌营养菌丝发育良好，老龄菌丝形成球状菌体。从营养菌丝分化出的孢子链含有不运动的杆状孢子。肽聚糖含有内消旋二氨基庚二酸和3-羟基二氨基庚二酸作为诊断性氨基酸。肽聚糖的酰基类型为糖基。在全细胞水解产物中检测到葡萄糖、甘露糖、核糖和木糖。主要的甲基萘醌是MK-10(H8)，其次是MK-10(H6)和MK-10(H4)。极性脂质谱由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇和磷脂酰肌醇甘露糖苷组成。主要脂肪酸为异-C15 : 0、异-C16 : 0和C17 : 1ω9c。基因组G+C含量为64.9mol%。基于16S rRNA基因序列的系统发育分析表明，菌株CPCC 204380应归入小单孢菌科，在该科中，它与根栖菌属、链孢囊菌属、球孢囊菌属、滨田菌属和异球孢囊菌属形成一个独特的分支。它与根栖菌Hellebori K12-0602（96.1%）、链孢囊菌Chokoriensis 2-25/1（95.9%）、球孢囊菌Koreensis DSM 44566（95.9%）、滨田菌Tsunoensis DSM 44101（95.3%）和异球孢囊菌Scoriae Sco-B14（94.2%）的16S rRNA基因序列相似度最高，与其他已有效描述的物种的序列相似度低于94.0%。系统发育分析和表型特征的结合支持将菌株CPCC 204380提议为小单孢菌科一个新属的新物种，为此提出新属名Allorhizocola rhizosphaerae gen. nov.，新种名Allorhizocola rhizosphaerae sp. nov.。CPCC 204380（=DSM 102292=KCTC 39746）是该模式种的模式菌株。
