Institute of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria.
Am J Trop Med Hyg. 2010 Mar;82(3):398-401. doi: 10.4269/ajtmh.2010.09-0417.
Validation of the sensitivity of the SYBR Green I in vitro test against an enzyme-linked immunosorbent assay (ELISA)-based drug sensitivity assay. Our results suggest that the SYBR Green I assay is a fast and inexpensive malaria drug screening assay for laboratory use. However, because of its lack of sensitivity in whole blood samples its usefulness for testing clinical samples may be limited.
验证 SYBR Green I 体外试验对基于酶联免疫吸附测定 (ELISA) 的药物敏感性测定的敏感性。我们的结果表明,SYBR Green I 测定法是一种快速且廉价的实验室用疟疾药物筛选测定法。然而,由于其在全血样本中的灵敏度不足,因此其用于测试临床样本的用途可能受到限制。