BiomaTiCS Research Group, Department of Ophthalmology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
Invest Ophthalmol Vis Sci. 2010 Aug;51(8):4047-53. doi: 10.1167/iovs.09-4759. Epub 2010 Mar 5.
This study was performed to investigate the surface topography of different glaucoma drainage devices and to determine the effects of surface roughness on cell adhesion of cultured human tenon fibroblasts.
The surface topography of four widely used devices (Ahmed FP7 and Ahmed S-2; New World Medical, Inc., Rancho Cucamonga, CA; Baerveldt BG101-350; Advanced Medical Optics, Irvine, CA; and Molteno S1; Molteno Ophthalmic Ltd., Dunedin, New Zealand) was investigated by scanning electron microscopy, and roughness was quantified by white-light confocal microscopy. Cells were grown for 72 hours on the surfaces of implants affixed to standard culture dishes. The cells were labeled with a fluorescent dye and detected by confocal laser scanning microscopy, while simultaneously imaging the surface reflectance. Collagen adsorption was quantified immunologically by using fluorescent beads coupled to a secondary antibody.
The root-mean-square roughness was 1.5 +/- 0.1 microm (mean +/- SE) for the silicone Ahmed model FP7 and 1.3 +/- 0.1 microm for the Ahmed with polypropylene base plate Ahmed model S-2. The Baerveldt was substantially smoother, with a mean roughness of 0.1 +/- 0.01 microm. The Molteno was the smoothest of all devices (0.07 +/- 0.01 microm). Cell adhesion was most prevalent on base plates with higher surface roughness, markedly less pronounced on the smoother base plates, and independent of collagen adsorption.
The most frequently implanted glaucoma drainage devices are of markedly different surface topography. Surface roughness appears to correlate with tenon fibroblast adhesion in vitro and also with the rate of occurrence of postimplantation hypertensive phase and failure due to fibrous encapsulation. Surface roughness may thus play a role in triggering excessive fibrovascular reactions. Smoother base plate surfaces may enhance the success rates of these devices.
本研究旨在观察不同青光眼引流装置的表面形貌，并确定表面粗糙度对培养的人眼Tenon 纤维细胞黏附的影响。
应用扫描电子显微镜观察四种广泛应用的青光眼引流装置（Ahmed FP7 和 Ahmed S-2；New World Medical，Rancho Cucamonga，CA；Baerveldt BG101-350；Advanced Medical Optics，Irvine，CA；Molteno S1；Molteno Ophthalmic Ltd.，Dunedin，New Zealand）的表面形貌，并应用白光共焦显微镜对表面粗糙度进行定量分析。将植入物固定在标准培养皿的表面，细胞在其上培养 72 小时。应用荧光染料对细胞进行标记，然后通过共焦激光扫描显微镜进行检测，同时对表面反射率进行成像。应用荧光珠与二抗偶联的方法对胶原蛋白吸附进行免疫定量分析。
硅酮 Ahmed 模型 FP7 的均方根粗糙度为 1.5 ± 0.1μm（平均值 ± SE），Ahmed 带聚丙烯底板模型 S-2 的均方根粗糙度为 1.3 ± 0.1μm。Baerveldt 则明显更光滑，平均粗糙度为 0.1 ± 0.01μm。所有装置中最光滑的是 Molteno（0.07 ± 0.01μm）。细胞黏附在表面粗糙度较高的底板上最普遍，在更光滑的底板上则显著减少，与胶原蛋白吸附无关。
最常植入的青光眼引流装置具有明显不同的表面形貌。表面粗糙度似乎与体外 Tenon 纤维细胞黏附相关，也与植入后高血压期和纤维包裹失败的发生率相关。因此，表面粗糙度可能在引发过度纤维血管反应中发挥作用。更光滑的底板表面可能会提高这些装置的成功率。
