Anselme K, Bigerelle M
Laboratoire de Recherche sur les Biomatériaux et les Biotechnologies, Université du Littoral Côte d'Opale, 52 rue du Dr Calot, 62608 Berck sur mer cedex, France.
Acta Biomater. 2005 Mar;1(2):211-22. doi: 10.1016/j.actbio.2004.11.009. Epub 2004 Dec 25.
Classically various treatments are applied to increase the roughness of titanium implants and improve their integration in the tissues. Many in vitro studies have been performed to better understand the mechanisms underlying the adhesion of cells on materials. Frequently, the adhesion is related to the attachment of cells during the first hours of contact with the substrate. For several years, our objective has been to develop experimental methods to evaluate the long-term adhesion of human osteoblasts from some hours to several weeks in order to model in vitro a tissue-like interface. This culture model allows for the formation over 21 days of a complex osteoblast/extracellular matrix/material interface. We recently developed a new parameter called adhesion power (AP) to evaluate this long-term adhesion. In this study, our objective is to check its efficiency in discriminating the long-term adhesion of human osteoblasts on pure titanium substrates with seven different surface morphologies obtained by electro-erosion, sandblasting, polishing, acid-etching and machine-tooling. By scanning electron microscopy, we observed that the human osteoblasts did spread more intimately on surface with low roughness amplitude than on rough ones. However, the AP was higher on rough isotropic surfaces obtained by electro-erosion, sandblasting or acid-etching and lower on smoother surfaces obtained by polishing and machine-tooling. We demonstrated that the AP was pertinent for evaluating human osteoblast's long-term adhesion on pure titanium surfaces with various roughness parameters. Its correlation with the order parameter, which describes the organization of the roughness, confirmed once more that human osteoblasts are more sensitive to the organization and morphology of the roughness than to its amplitude.
传统上，人们采用各种处理方法来增加钛植入物的粗糙度，并改善其在组织中的整合。已经进行了许多体外研究，以更好地理解细胞在材料上粘附的潜在机制。通常，粘附与细胞在与底物接触的最初几个小时内的附着有关。几年来，我们的目标是开发实验方法，以评估人类成骨细胞从数小时到数周的长期粘附，以便在体外模拟组织样界面。这种培养模型允许在21天内形成复杂的成骨细胞/细胞外基质/材料界面。我们最近开发了一个名为粘附力（AP）的新参数来评估这种长期粘附。在本研究中，我们的目标是检验其在区分人类成骨细胞在通过电蚀、喷砂、抛光、酸蚀和机械加工获得的七种不同表面形态的纯钛基板上的长期粘附方面的效率。通过扫描电子显微镜，我们观察到人类成骨细胞在粗糙度幅度低的表面上比在粗糙表面上更紧密地铺展。然而，通过电蚀、喷砂或酸蚀获得的粗糙各向同性表面上的AP较高，而通过抛光和机械加工获得的较光滑表面上的AP较低。我们证明了AP对于评估人类成骨细胞在具有各种粗糙度参数的纯钛表面上的长期粘附是相关的。它与描述粗糙度组织的有序参数的相关性再次证实，人类成骨细胞对粗糙度的组织和形态比对其幅度更敏感。
