The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Jiangsu, China.
Appl Environ Microbiol. 2010 May;76(9):2989-96. doi: 10.1128/AEM.00026-09. Epub 2010 Mar 5.
Lactobacillus sanfranciscensis DSM20451 cells containing glutathione (GSH) displayed significantly higher resistance against cold stress induced by freeze-drying, freeze-thawing, and 4 degrees C cold treatment than those without GSH. Cells containing GSH were capable of maintaining their membrane structure intact when exposed to freeze-thawing. In addition, cells containing GSH showed a higher proportion of unsaturated fatty acids in cell membranes upon long-term cold treatment. Subsequent studies revealed that the protective role of GSH against cryodamage of the cell membrane is partly due to preventing peroxidation of membrane fatty acids and protecting Na(+),K(+)-ATPase. Intracellular accumulation of GSH enhanced the survival and the biotechnological performance of L. sanfranciscensis, suggesting that the robustness of starters for sourdough fermentation can be improved by selecting GSH-accumulating strains. Moreover, the results of this study may represent a further example of mechanisms for stress responses in lactic acid bacteria.
含有谷胱甘肽 (GSH) 的旧金山乳杆菌 DSM20451 细胞在冻干、冻融和 4°C 冷藏处理引起的冷应激下表现出明显更高的抗性，比不含 GSH 的细胞高。含有 GSH 的细胞在暴露于冻融时能够保持其膜结构完整。此外，含有 GSH 的细胞在长期冷藏处理时，细胞膜中的不饱和脂肪酸比例更高。随后的研究表明，GSH 对细胞膜冷冻损伤的保护作用部分归因于防止膜脂肪酸的过氧化和保护 Na(+),K(+)-ATP 酶。细胞内 GSH 的积累增强了旧金山乳杆菌的生存能力和生物技术性能，表明通过选择积累 GSH 的菌株可以提高发酵剂的鲁棒性。此外，本研究的结果可能代表了乳酸菌应激反应机制的另一个例子。
