Melchior T, Hallam D, Pedersen N T
Medical Department, Municipal Hospital, Copenhagen, Denmark.
Scand J Clin Lab Invest. 1991 Feb;51(1):85-91.
A method is described for measuring the potassium content in leukocytes using Percoll (R) density gradient centrifugation. Ninety subjects between 21 and 92 years formed the reference population. The magnesium content in leukocytes could not be estimated because of interaction between the ion and the Percoll (R) media. Sex, age, leukocytosis because of infection, physical stress, venous stasis did not interfere with the analysis. The potassium content was calculated per cell and per g-1 of DNA. The granulocyte potassium content was (median (range)) 37.4 (25.8-75.0) fmol/cell-1 or 2.9 (1.5-9.8) mmol g-1 DNA. The lymphocyte potassium content was 45.9 (26.4-69.6) fmol cell-1 or 3.3 (1.5-5.0) mmol g-1 DNA. The coefficient of variation (less than 10%) was not reduced by using cell DNA instead of cell number as reference. The interindividual variation was high, making the test unfit for clinical use. The leukocyte potassium content was not decreased in patients with acute myocardial infarction nor in patients treated with diuretics and potassium supplements because of cardiovascular disease.
描述了一种使用Percoll（R）密度梯度离心法测量白细胞中钾含量的方法。90名年龄在21至92岁之间的受试者构成了参考人群。由于离子与Percoll（R）介质之间的相互作用，无法估算白细胞中的镁含量。性别、年龄、因感染、身体应激、静脉淤滞导致的白细胞增多均不影响分析。钾含量按每个细胞和每克DNA计算。粒细胞钾含量为（中位数（范围））37.4（25.8 - 75.0）fmol/细胞或2.9（1.5 - 9.8）mmol/g DNA。淋巴细胞钾含量为45.9（26.4 - 69.6）fmol/细胞或3.3（1.5 - 5.0）mmol/g DNA。使用细胞DNA而非细胞数量作为参考，变异系数（小于10%）并未降低。个体间差异很大，使得该检测不适用于临床。急性心肌梗死患者以及因心血管疾病接受利尿剂和钾补充剂治疗的患者，其白细胞钾含量并未降低。
