Nagashima Yuji, Mataki Isao, Toyoda Maho, Nakajima Hiroshi, Tsumoto Kingo, Shimakura Kuniyoshi, Shiomi Kazuo
Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan.
Shokuhin Eiseigaku Zasshi. 2010;51(1):48-51. doi: 10.3358/shokueishi.51.48.
Changes in tetrodotoxin (TTX) content of the puffer fish Takifugu rubripes during seed production were examined. Two mature female puffer fish T. rubripes (samples 1 and 2) that contained TTX were used. The toxic eggs were artificially fertilized, and hatchlings were reared in an indoor tank for 50 days and then in a netcage at sea for an additional 48 or 38 days. The TTX content of the fertilized eggs of sample 1 was initially 13.0 microg TTX/g, transiently increased to 67.6 microg TTX/g at 4 days after hatching, and then gradually decreased to 0.28 microg TTX/g at 98 days. In contrast, the total TTX content in an individual was 0.016 microg TTX at the fertilization stage and 0.01-0.03 microg TTX at the larval stage until 30 days after hatching. Thereafter, the total TTX content increased remarkably during culture in the netcage at sea, reaching 4.80 microg TTX at 98 days. Change in the TTX content of sample 2 showed a similar tendency to that of sample 1. The present study showed that the TTX content per gram of puffer fish body weight decreased during progression from fertilized eggs to juveniles, whereas the total TTX content increased.
研究了红鳍东方鲀在育苗过程中河豚毒素（TTX）含量的变化。使用了两条含有TTX的成熟雌性红鳍东方鲀（样本1和样本2）。对有毒鱼卵进行人工授精，幼鱼在室内水箱中饲养50天，然后在海上网箱中再饲养48天或38天。样本1受精卵的TTX含量最初为13.0微克TTX/克，孵化后4天短暂增加到67.6微克TTX/克，然后在98天时逐渐降至0.28微克TTX/克。相比之下，个体在受精阶段的总TTX含量为0.016微克TTX，在孵化后30天内的幼体阶段为0.01 - 0.03微克TTX。此后，在海上网箱养殖期间总TTX含量显著增加，在98天时达到4.80微克TTX。样本2的TTX含量变化与样本1相似。本研究表明，从受精卵发育到幼鱼的过程中，每克河豚鱼体重的TTX含量下降，而总TTX含量增加。
