Panagene Inc., Daejeon 305-510, Korea.
J Microbiol Biotechnol. 2010 Feb;20(2):287-93.
Reliable discrimination of single nucleotide mismatch was demonstrated using arrays with peptide nucleic acid (PNA) probes. Newly developed PNA probes immobilization method and hybridization conditions for PNA arrays gave excellent specificity and sensitivity. And we compared the specificity, sensitivity, and stability obtained with the PNA and DNA arrays in discriminating single nucleotide mismatches. PNA arrays had superior perfect match-to-mismatch signal ratios and sensitivities. The relative signal intensities of mismatch PNA probes ranged from 1.6% to 12.1% of the perfect match PNA probes. These results demonstrated that the PNA arrays were 2.0 to 37.3 times more specific and about 10 times more sensitive than DNA arrays. A PNA array showed the same specificity and sensitivity after 12-month storage at room temperature.
使用肽核酸 (PNA) 探针的阵列可靠地区分单核苷酸错配。新开发的 PNA 探针固定化方法和 PNA 阵列的杂交条件提供了极好的特异性和灵敏度。我们比较了 PNA 和 DNA 阵列在区分单核苷酸错配方面的特异性、灵敏度和稳定性。PNA 阵列具有优越的完美匹配与错配信号比率和灵敏度。错配 PNA 探针的相对信号强度范围为完美匹配 PNA 探针的 1.6%至 12.1%。这些结果表明，PNA 阵列比 DNA 阵列更具特异性和灵敏度，其特异性和灵敏度分别提高了 2.0 至 37.3 倍和约 10 倍。PNA 阵列在室温下储存 12 个月后仍具有相同的特异性和灵敏度。
