Zhang Yanhua, Tang Yuping, Guo Jianming, Ding Anwei, Duan Jinao
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(21):2821-6.
To investigate the effect of five traditional Chinese medicine formula for promoting blood circulation to remove stasis (Xuefuzhuyu Tang, Shaofuzhuyu Tang, Gexiazhuyu Tang, ShentongZhuyu Tang, Tongqiaohuoxue Tang) on platelet aggregation and clotting time in a dose-response relationship.
The platelet aggregation was tested with Born's method and thrombin time (TT) method was established to determine the clotting time.
The formula for promoting blood circulation to remove stasis showed significant inhibitory effects on the platelet aggregation and prolonged clotting time, the supernate of 80% alcohol solution showed the most potent inhibitory activity among the three kinds of samples from one formula (P < 0.01), especially the supernate of Shaofuzhuyu Tang and Gexiazhuyu Tang were better than other formula on platelet aggregation. About the anticoagulant, the whole formula showed the evidently prolonging the clotting time than other two fractions (P < 0.01) and the precipitation were the worst.
The formula for promoting blood circulation to remove stasis showed significant effect on platelet aggregation and clotting time, and different samples of the formula had different efficacy. The study established a foundation for further bio-activity evaluation of these formulae and provided evidence for the treatment of cardiovascular and cerebrovascular diseases.
探讨血府逐瘀汤、少腹逐瘀汤、膈下逐瘀汤、身痛逐瘀汤、通窍活血汤这五首活血化瘀类中药方剂对血小板聚集及凝血时间的量效关系。
采用玻恩氏法检测血小板聚集情况，并建立凝血酶时间（TT）法测定凝血时间。
活血化瘀类方剂对血小板聚集有显著抑制作用，且延长凝血时间，同一方剂的三种样品中80%乙醇溶液上清液的抑制活性最强（P<0.01），尤其是少腹逐瘀汤和膈下逐瘀汤的上清液对血小板聚集的抑制作用优于其他方剂。在抗凝方面，全方对凝血时间的延长作用明显优于其他两个部位（P<0.01），沉淀部分效果最差。
活血化瘀类方剂对血小板聚集和凝血时间有显著影响，且方剂的不同样品疗效不同。本研究为进一步评价这些方剂的生物活性奠定了基础，为心脑血管疾病的治疗提供了依据。
