[蒙古黄芪不同提取物的抗氧化活性]

[Antioxidant activity of different extracts from Astragalus mongholicus].

作者信息

Bian Yunyun, Li Ping

机构信息

Key Laboratory of Modern Chinese Medicines, Ministry of Education and Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(22):2924-7.

PMID:20209962

Abstract

OBJECTIVE

To study the antioxidant activities of different extracts from Astragalus mongholicus.

METHOD

The antioxidant activities of the total flavanoids (TFA), the total saponins (TSA) and the total polysaccharides (TPA) from A. mongholicus were measured by means of biochemiluminescence. The concentrations of these samples varied from 1.25 x 10(-3) g x L(-1) to 25 x10(-3) g x L(-1).

RESULT

All the extracts showed antioxidant activities of scavenging 3 different free radicals with obvious concentration-scavenging capacity relationship. The antioxidant activity of TFA was the strongest among them. The IC50 of scavenging superoxide anion radicals of TFA (55.48% TFA, 0.59% TFA and 0.36% TFA) were 0.654 2, 4.654 4 and 6.055 2 g x L(-1), the IC50 of scavenging hydroxyl anion were 0.060 5, 0.254 4 and 0.493 0 g x L(-1), and the IC50 of scavenging hydrogen peroxide were 0.010 4, 0.156 0 and 0.224 0 g x L(-1), respectively.

CONCLUSION

The extracts are more sensitive to hydrogen peroxide and hydroxyl anion than to superoxide anion radicals. TFA of A. mongholicus Bunge is the main component responsible for antioxidant activity, while TSA and TPA have weaker antioxidant activity. The antioxidant activity of TFA increases with the rise of its purity. On the contrary, the antioxidant activity of TSA and TPA decreases with the rise of their purity.

摘要

目的

研究蒙古黄芪不同提取物的抗氧化活性。

方法

采用生物发光法测定蒙古黄芪中总黄酮（TFA）、总皂苷（TSA）和总多糖（TPA）的抗氧化活性。这些样品的浓度范围为1.25×10⁻³ g·L⁻¹至25×10⁻³ g·L⁻¹。

结果

所有提取物均表现出对3种不同自由基的清除抗氧化活性，且具有明显的浓度-清除能力关系。其中TFA的抗氧化活性最强。TFA清除超氧阴离子自由基的IC50（55.48% TFA、0.59% TFA和0.36% TFA）分别为0.654 2、4.654 4和6.055 2 g·L⁻¹，清除羟阴离子的IC50分别为0.060 5、0.254 4和0.493 0 g·L⁻¹，清除过氧化氢的IC50分别为0.010 4、0.156 0和0.224 0 g·L⁻¹。