Altun Mehmet Levent, Citoğlu Gülcin Saltan, Yilmaz Betül Sever, Coban Tülay
Department of Pharmacognosy, Faculty of Pharmacy, University of Ankara, Tandogan, Ankara, Turkey.
Int J Food Sci Nutr. 2008 May;59(3):175-80. doi: 10.1080/09637480701381648.
In the present study, antioxidant properties of the water extracts of different parts of Viburnum opulus and Viburnum lantana (Caprifoliaceae) were investigated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and superoxide anion scavenging methods. The extracts were prepared from the fruits, branches, and leaves of V lantana and V opulus species. The branch extracts of V lantana and V opulus inhibited superoxide anion in a concentration-dependent manner. Compared with t-tocopherol, the fruit extract of V lantana did not show any scavenging effect on superoxide anion formation. V lantana leaf extracts, however, showed a moderate scavenging effect on superoxide anion formation, whereas V lantana branch extracts showed a strong scavenging effect (IC50 = 3.1 mg/ml) on superoxide anion in higher concentration. On the other hand, all extracts exhibited a scavenging effect on the DPPH radical with various potencies. When compared with butylated hydroxytoluene, V opulus branch and V lantana leaf extracts, as well as V lantana branch, V opulus fruit and V lantana fruit extracts, showed strong DPPH radical scavenging activity with IC50 values of 0.014, 0.035, 0.052, 0.057 and 0.085 mg/ml, respectively.
在本研究中,使用2,2-二苯基-1-苦基肼(DPPH)清除法和超氧阴离子清除法,研究了欧洲荚蒾和南欧荚蒾(忍冬科)不同部位水提取物的抗氧化特性。提取物分别从南欧荚蒾和欧洲荚蒾的果实、枝条和叶子中制备。南欧荚蒾和欧洲荚蒾的枝条提取物以浓度依赖的方式抑制超氧阴离子。与生育酚相比,南欧荚蒾的果实提取物对超氧阴离子的形成没有任何清除作用。然而,南欧荚蒾叶提取物对超氧阴离子的形成表现出中等的清除作用,而南欧荚蒾枝条提取物在较高浓度下对超氧阴离子表现出较强的清除作用(IC50 = 3.1毫克/毫升)。另一方面,所有提取物对DPPH自由基均表现出不同程度的清除作用。与丁基羟基甲苯相比,欧洲荚蒾枝条提取物、南欧荚蒾叶提取物以及南欧荚蒾枝条、欧洲荚蒾果实和南欧荚蒾果实提取物对DPPH自由基均表现出较强的清除活性,IC50值分别为0.014、0.035、0.052、0.057和0.085毫克/毫升。