Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
School of Pharmacy, Ningxia Medical University, Yinchuan 750021, China.
Molecules. 2019 Jan 25;24(3):434. doi: 10.3390/molecules24030434.
The root of var. is one of the most popular herbal medicines worldwide. In order to increase the yield of underground roots of var. , its flowers (AMF) have often been removed in their flowering stage, which produces the flowers as waste being discarded. To explore its phytochemicals and potential value for utilization, the antioxidant activities of extracts from AMF were evaluated by a free radical scavenging assay and reducing power assay. The total phenols and flavonoids, as well as the individual compounds, in different extracts of AMF were also investigated. The results showed that the extract ME obtained from AMF through macroporous resins separation exhibited strong antioxidant activities, which were close to those of positive control BHT. ME was rich in phenolic acids and flavonoids, and the contents reached 108.42 mg gallic acid equivalents/g and 265.70 mg rutin equivalents/g, respectively. A total of 31 compounds, including four phenolic acids, nineteen flavonoids, three isoflavones, two pterocarpans, and three saponins, were identified using UPLC-QTOF-MS in ME. Quantitative analysis of sixteen components in the extracts of AMF showed that flavonoids were the predominant constituents, especially for the compounds of hyperoside, rutin, and isorhamnetin-3-O-β-d-glucoside.
var. 的根是全球最受欢迎的草药之一。为了提高地下根的产量，通常会在开花期去除其花（AMF），将花作为废物丢弃。为了探索其植物化学物质和潜在的利用价值，通过自由基清除测定法和还原力测定法评估了 AMF 提取物的抗氧化活性。还研究了 AMF 不同提取物中的总酚类和类黄酮以及个别化合物。结果表明，通过大孔树脂分离从 AMF 获得的提取物 ME 表现出很强的抗氧化活性，与阳性对照 BHT 相当。ME 富含酚酸和类黄酮，含量分别达到 108.42 毫克没食子酸当量/克和 265.70 毫克芦丁当量/克。使用 UPLC-QTOF-MS 在 ME 中鉴定了 31 种化合物，包括四种酚酸、十九种类黄酮、三种异黄酮、两种紫檀烷和三种皂苷。对 AMF 提取物中十六种成分的定量分析表明，类黄酮是主要成分，尤其是化合物金丝桃苷、芦丁和异鼠李素-3-O-β-d-葡萄糖苷。
