Yin Gang, Xu Zhi, Xu Yi-Zhuang, Sun Qi-Gang, Liu Ya-Qi, Zhang Yuan-Fu, Wu Jin-Guang, Zhou Xiao-Si, Ling Xiao-Feng
Department of General Surgery, Peking University Third Hospital, Beijing 100191, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Dec;29(12):3241-5.
To explore the feasibility of quick intraoperative in situ and noninvasive diagnosis of cholangiocarcinoma by Fourier transform infrared (FTIR) spectroscopy, 26 samples of freshly resected cholangiocarcinoma tissues and 43 samples of benign bile duct tissue were collected during surgery. And all fresh samples were measured by FTIR spectroscopy via probe of attenuated total reflection (ATR) without pretreatment immediately after resection. For each spectrum, 12 bands were identified and assigned between 3 800 and 1 000 cm(-1). The peak position was found, and the intensity and area of the bands were measured; The ratios of the intensity and area were calculated. Standard statistic analysis was performed. The results illustrated that the FTIR spectra of malignant tissue were significantly different from those of benign tissue: 1. The bands related to lipid: The peak of 2 925 cm(-1) shifted to lower wave number significantly (P = 0.033); 2. The bands related to nucleic acid and lipids: the ratios of I1 083/ I1 460 (P = 0.005), S1 083 /S1 460 (P = 0.001) and S1 240/S1 460 (P = 0.025) raised significantly, indicating that the relative quantity of nucleic acid to lipids had evidently increased in malignancy. 3. The bands related to protein: The ratios of I1 550/I1 083 (P = 0.000) and S1 550/S1 083 (P = 0.000) reduced significantly, implying that the relative quantity of protein to nucleic acid in malignancy decreased significantly and the secondary structure of protein changed probably. Our primary result illustrated that the FTIR spectroscopy technique maybe a promising method for in situ and quick intraoperative diagnosis of cholangiocarcinoma and has great value for clinical application.
为探讨傅里叶变换红外（FTIR）光谱技术在术中快速原位无创诊断胆管癌的可行性，手术中收集了26例新鲜切除的胆管癌组织样本和43例良性胆管组织样本。所有新鲜样本在切除后未经预处理，立即通过衰减全反射（ATR）探头进行FTIR光谱检测。对每个光谱，在3800至1000 cm⁻¹之间识别并指定了12条谱带。确定了峰位置，测量了谱带的强度和面积；计算了强度和面积的比值。进行了标准统计分析。结果表明，恶性组织的FTIR光谱与良性组织有显著差异：1. 与脂质相关的谱带：2925 cm⁻¹处的峰明显向低波数移动（P = 0.033）；2. 与核酸和脂质相关的谱带：I1083/I1460（P = 0.005）、S1083/S1460（P = 0.001）和S1240/S1460（P = 0.025）的比值显著升高，表明恶性肿瘤中核酸与脂质的相对含量明显增加。3. 与蛋白质相关的谱带：I1550/I1083（P = 0.000）和S1550/S1083（P = 0.000）的比值显著降低，这意味着恶性肿瘤中蛋白质与核酸的相对含量显著降低，蛋白质的二级结构可能发生了变化。我们的初步结果表明，FTIR光谱技术可能是一种有前景的术中胆管癌原位快速诊断方法，具有重要的临床应用价值。
