Liu Ya-Qi, Gao Mei-Juan, Xu Yi-Zhuang, Sun Qi-Gang, Zhang Xiao-Qing, Xu Zhi, Zhang Yuan-Fu, Wu Jin-Guang, Zhou Xiao-Si, Ling Xiao-Feng
Department of General Surgery, Third Hospital of Peking University, Beijing 100191, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Nov;29(11):2917-21.
The aim of the study is to explore the possibility of detecting metastatic lymph nodes by FTIR spectra during the surgery of thyroid cancer. The FTIR spectra of 20 metastatic lymph nodes and 69 non-metastatic ones were collected via an ATR (attenuated total reflectance) probe. For each spectrum, 28 variables of 13 bands including peak positions and relative intensities were measured. The variables of metastatic lymph node were compared to those of non-metastatic ones using standard statistic methods. The results indicated that the FTIR spectra of metastatic lymph nodes were significantly different from non-metastatic ones in the bands related to protein, lipid, nucleic acid and carbohydrate. (1) Variations of bands related to protein: The relative intensity ratios of I3 280 /I1 460, I1 640 /I1 460 and I1 546/I1 460 increased significantly (P < 0.05); (2) Variations of bands related to lipid: The relative intensity ratios of I1 743 /I1 460 decreased significantly (P < 0.05). On the contrary, I 400 /I1 460 increased significantly (P < 0.05); (3) The peak positions of 1 165 and 1 120 cm(-1) which were all assigned to carbohydrate shifted toward higher wave number (P < 0.05). The relative intensity ratio of I1 165 /I1 460 decreased significantly (P < 0.05); (4) The peak positions of 1 085 cm(-1) related to nucleic acid shifted to the lower wave number (P < 0.05); (5) Other undetermined bands: The relative intensity ratios of I1 303 /I1 460 and I1 303 /I1 240 increased significantly (P < 0.05). FTIR spectroscopy could be a reliable and practicable method for metastatic lymph nodes diagnosis in the operation of thyroid cancer. It could be applied in detecting metastatic lymph nodes which can not be determined by palpation in surgery.
本研究的目的是探索在甲状腺癌手术过程中通过傅里叶变换红外光谱（FTIR）检测转移性淋巴结的可能性。通过衰减全反射（ATR）探头收集了20个转移性淋巴结和69个非转移性淋巴结的FTIR光谱。对于每个光谱，测量了13个波段的28个变量，包括峰位和相对强度。使用标准统计方法将转移性淋巴结的变量与非转移性淋巴结的变量进行比较。结果表明，转移性淋巴结的FTIR光谱在与蛋白质、脂质、核酸和碳水化合物相关的波段中与非转移性淋巴结有显著差异。（1）与蛋白质相关波段的变化：I3280/I1460、I1640/I1460和I1546/I1460的相对强度比显著增加（P<0.05）；（2）与脂质相关波段的变化：I1743/I1460的相对强度比显著降低（P<0.05）。相反，I400/I1460显著增加（P<0.05）；（3）均归属于碳水化合物的1165和1120cm-1的峰位向更高波数移动（P<0.05）。I1165/I1460的相对强度比显著降低（P<0.05）；（4）与核酸相关的1085cm-1的峰位向更低波数移动（P<0.05）；（5）其他未确定的波段：I1303/I1460和I1303/I1240的相对强度比显著增加（P<0.05）。FTIR光谱可能是甲状腺癌手术中诊断转移性淋巴结的一种可靠且实用的方法。它可用于检测手术中触诊无法确定的转移性淋巴结。
