Sun Qi-Gang, Liu Ya-Qi, Xu Yi-Zhuang, Zhang Yuan-Fu, Wu Jin-Guang, Zhou Xiao-Si, Xu Zhi, Ling Xiao-Feng
Department of General Surgery, Peking University Third Hospital, Beijing 100191, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2010 Dec;30(12):3195-8.
Fourier transform infrared (FTIR) spectroscopy with an attenuated total reflection(ATR) probe was used to study the benign and malignant tissues of gallbladder. FTIR spectra of 18 tissues of gallbladder carcinoma and 139 benign tissues were collected and analyzed. The results demonstrated that bands related to lipid, carbohydrate, and nucleic acid from malignant samples were significantly different from those of benign ones: (1) The peak positions of 1 167 and 1 123 cm(-1) shifted toward lower wave number (P < 0.05) but that of 1 309 cm(-1)' to the higher one (P < 0.05); (2) The relative intensity ratios of I2 856/I1 461, I1 167/ I1 461, I1 123/I1 461, I1 082/I1 461, increased significantly (P < 0.05) (3) The half-widths of 1 167 and 1 082 cm(-1) increased significantly (P < 0.05), whereas the half-width of 1 461 cm(-1) decreased significantly (P < 0.05). (4) The probability of 1 750 cm(-1) band of gallbladder carcinoma tissues increased significantly (P < 0.05).
采用带有衰减全反射（ATR）探头的傅里叶变换红外（FTIR）光谱技术对胆囊的良性和恶性组织进行研究。收集并分析了18例胆囊癌组织和139例良性组织的FTIR光谱。结果表明，恶性样本中与脂质、碳水化合物和核酸相关的谱带与良性样本有显著差异：（1）1167和1123 cm⁻¹处的峰位向低波数移动（P < 0.05），而1309 cm⁻¹处的峰位向高波数移动（P < 0.05）；（2）I2856/I1461、I1167/I1461、I1123/I1461、I1082/I1461的相对强度比显著增加（P < 0.05）；（3）1167和1082 cm⁻¹处的半高宽显著增加（P < 0.05），而1461 cm⁻¹处的半高宽显著减小（P < 0.05）；（4）胆囊癌组织在1750 cm⁻¹处谱带出现的概率显著增加（P < 0.05）。
