University of Technology Dresden, Medical Faculty, Clinical Sensoring and Monitoring, Dresden, Germany.
J Biomed Opt. 2010 Jan-Feb;15(1):016030. doi: 10.1117/1.3302809.
In vivo determination of 3-D and dynamic geometries of alveolar structures with adequate resolution is essential for developing numerical models of the lung. A thorax window is prepared in anesthetized rabbits by removal of muscle tissue between the third and fourth rib without harming the parietal pleura. The transparent parietal pleura allows contact-free imaging by intravital microscopy (IVM) and 3-D optical coherence tomography (3-D OCT). We demonstrate that dislocation of the lung surface is small enough to observe identical regions in the expiratory and inspiratory plateau phase, and that OCT in this animal model is suitable for generating 3-D geometry of in vivo lung parenchyma. To our knowledge, we present a novel thorax window preparation technique for 3-D imaging of alveolar dynamics for the first time. The 3-D datasets of the fine structure of the lung beneath the pleura could provide a basis for the development of 3-D numerical models of the lung.
用足够分辨率的活体方法来确定肺泡结构的 3D 和动态几何形状，对于开发肺部的数值模型至关重要。通过去除第三和第四肋骨之间的肌肉组织而不损伤壁层胸膜，在麻醉的兔子中制备胸腔窗。透明的壁层胸膜允许通过活体显微镜（IVM）和 3D 光学相干断层扫描（3D-OCT）进行无接触成像。我们证明，肺表面的移位小到足以在呼气和吸气平台阶段观察相同的区域，并且该动物模型中的 OCT 适合于生成活体肺实质的 3D 几何形状。据我们所知，我们首次提出了一种用于肺泡动力学 3D 成像的新型胸腔窗制备技术。胸膜下肺的精细结构的 3D 数据集可为肺部的 3D 数值模型的开发提供基础。
