海藻酸钠作为一种固定化材料，用于通过包埋杂交瘤细胞生产单克隆抗体。

Alginate as an immobilization material for MAb production via encapsulated hybridoma cells.

机构信息

Department of Bioengineering, Ege University, Bornova, Izmir, Turkey.

出版信息

Crit Rev Biotechnol. 2010 Jun;30(2):145-59. doi: 10.3109/07388550903451652.

PMID:20210690

Abstract

Alginate has been widely used in various applications since its first extraction. What makes this biopolymer useful is its high biocompatibility and humid gelation conditions. Both of these features bring it into prominence as an ideal immobilization material. However, there are some complicated aspects of cell immobilization using alginate biopolymers. This review discusses and clarifies these crucial points, using as an example the bioprocessing of highly fragile cells (hybridoma cells). The review focuses on the cultivation and production of alginate encapsulated cells.

摘要

藻酸盐自首次提取以来，已广泛应用于各种领域。使其成为有用物质的原因是其具有高度的生物相容性和潮湿的凝胶条件。这两个特点使它成为一种理想的固定化材料。然而，使用藻酸盐生物聚合物进行细胞固定化存在一些复杂的方面。本综述以高度脆弱的细胞（杂交瘤细胞）的生物加工为例，讨论并阐明了这些关键点。本综述重点介绍了藻酸盐包埋细胞的培养和生产。