Department of Chemistry, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, Croatia.
Biotechnology Department, M. Auezov South-Kazakhstan University, Tauke-Khan, 160000 Shymkent, Kazakhstan.
Int J Mol Sci. 2021 Feb 12;22(4):1847. doi: 10.3390/ijms22041847.
Novel plant growth regulators (PGRs) based on the derivatives of dehydroamino acids 2,3-dehydroaspartic acid dimethyl ester (PGR1), Z-isomer of the potassium salt of 2-amino-3-methoxycarbonylacrylic acid (PGR2) and 1-methyl-3-methylamino-maleimide (PGR3) have been synthesized and their growth-regulating properties investigated. Laboratory testing revealed their plant growth-regulating activity. PGR1 showing the most stimulating activity on all laboratory tested cultures were used in field experiments. Results showed that PGR1 is a highly effective environmentally friendly plant growth regulator with effects on different crops. Biopolymeric microcapsule formulations (chitosan/alginate microcapsule loaded with PGR) suitable for application in agriculture were prepared and characterized. Physicochemical properties and release profiles of PGRs from microcapsule formulations depend on the molecular interactions between microcapsule constituents including mainly electrostatic interactions and hydrogen bonds. The differences in the microcapsule formulations structure did not affect the mechanism of PGRs release which was identified as diffusion through microcapsules. The obtained results opened a perspective for the future use of microcapsule formulations as new promising agroformulations with a sustained and target release for plant growth regulation.
新型植物生长调节剂（PGRs）基于去氢氨基酸 2,3-脱氢天冬氨酸二甲酯（PGR1）、2-氨基-3-甲氧羰基丙烯酸的钾盐 Z-异构体（PGR2）和 1-甲基-3-亚氨基马来酰亚胺（PGR3）的衍生物合成，并研究了它们的生长调节特性。实验室测试显示了它们的植物生长调节活性。在所有实验室测试的培养物中，PGR1 表现出最刺激的活性，因此被用于田间试验。结果表明，PGR1 是一种高效、环保的植物生长调节剂，对不同作物都有效果。制备并表征了适用于农业应用的生物聚合物微胶囊制剂（负载 PGR 的壳聚糖/海藻酸盐微胶囊）。微胶囊制剂中 PGR 的理化性质和释放特性取决于微胶囊成分之间的分子相互作用，主要包括静电相互作用和氢键。微胶囊制剂结构的差异并不影响 PGR 释放的机制，确定为通过微胶囊扩散。这些结果为未来使用微胶囊制剂作为具有持续和靶向植物生长调节释放的新型有前途的农用制剂开辟了前景。
