Laremore Tatiana N, Leach Franklin E, Amster I Jonathan, Linhardt Robert J
Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 110 Eighth Street, Troy, New York 12180, USA.
Int J Mass Spectrom. 2011 Aug 15;305(2-3):109-115. doi: 10.1016/j.ijms.2010.09.020.
A mixture of glycosaminoglycan (GAG) chains from a plasma proteoglycan bikunin was fractionated using native, continuous-elution polyacrylamide gel electrophoresis, and the resulting fractions were analyzed by electrospray ionization Fourier transform mass spectrometry (ESI FTMS). Molecular mass analysis of the intact GAG afforded information about the length and composition of GAG chains in the mixture. Ambiguity in the interpretation of the intact GAG mass spectra was eliminated by conducting an additional experiment in which the GAG chains of known molecular mass were treated with a GAG-degrading enzyme, chondroitinase ABC, and the digestion products were analyzed by ESI FTMS. The plasma bikunin GAG chains consisted predominantly of odd number of saccharides, although few chains consisting of even number of saccharides were also detected. Majority of the analyzed chains were tetrasulfated or pentasulfated and comprised by 29 to 41 monosaccharides.
使用天然连续洗脱聚丙烯酰胺凝胶电泳对血浆蛋白聚糖比基尼的糖胺聚糖（GAG）链混合物进行分级分离，然后通过电喷雾电离傅里叶变换质谱（ESI FTMS）对所得级分进行分析。完整GAG的分子量分析提供了混合物中GAG链长度和组成的信息。通过进行一项额外实验消除了完整GAG质谱解释中的模糊性，在该实验中，用GAG降解酶软骨素酶ABC处理已知分子量的GAG链，并通过ESI FTMS分析消化产物。血浆比基尼GAG链主要由奇数个糖类组成，不过也检测到少数由偶数个糖类组成的链。大多数分析的链是四硫酸化或五硫酸化的，由29至41个单糖组成。
