Mt Sinai Medical Center, Division Neonatology, Miami Beach, FL 33140, USA.
Life Sci. 2010 May 8;86(19-20):707-15. doi: 10.1016/j.lfs.2010.02.022. Epub 2010 Mar 6.
Periodic acceleration (pGz) is a method that applies repetitive sinusoidal head-to-foot motion to the horizontally positioned body. pGz adds pulses to the circulation as a function of frequency, thereby increasing shear stress to the endothelium. Pulsatile shear stress increases release of cardioprotective endothelial-derived nitric oxide prostaglandin E-2 and prostacyclin into the circulation. We investigated whether pGz may be effective as an early preconditioning strategy when applied one hour prior to whole body ischemia reperfusion injury induced by ventricular fibrillation (VF).
Twenty anesthetized and paralyzed male swine were randomized to one hour of pGz and conventional mechanical ventilation [PC] or solely conventional mechanical ventilation [Control] prior to VF and resuscitation. After eight minutes of unsupported VF, cardiopulmonary resuscitation was carried out followed by defibrillation. Hemodynamics, electrocardiogram, echocardiogram, regional blood flows, and markers of global myocardial injury were measured. Protein expression of endothelial-derived nitric oxide synthase (eNOS), phosphorylated eNOS (p-eNOS), serine/threonine kinase Akt total (t-Akt), and phosphorylated (p-Akt) were determined by immunoblotting.
All animals had spontaneous return of circulation after cardiopulmonary resuscitation (CPR) and defibrillation. Preconditioned animals had less hemodynamically significant arrhythmias, less myocardial stunning, and greater regional blood flows to the brain, heart, kidneys, and ileum than Controls. Troponin I and creatine phosphokinase values in PC were 65% of the values present in Controls. In addition, preconditioned animals had higher protein expression of cardiac eNOS, p-eNOS, t-Akt, and p-Akt than Controls.
pGz preconditioning confers early cardioprotection in a model of whole body ischemia reperfusion injury.
周期性加速度（pGz）是一种将水平放置的身体进行头到脚的重复正弦运动的方法。pGz 通过频率为循环增加脉冲，从而增加内皮细胞的剪切应力。脉动剪切应力增加了内皮衍生的保护性一氧化氮前列腺素 E-2 和前列环素释放到循环中。我们研究了 pGz 是否可以作为一种早期预处理策略，在心室颤动（VF）引起的全身缺血再灌注损伤前一小时应用。
20 只麻醉和麻痹的雄性猪随机分为 pGz 和常规机械通气[PC]或仅常规机械通气[对照]一小时，然后进行 VF 和复苏。在无支持的 VF 八分钟后，进行心肺复苏，然后除颤。测量血流动力学、心电图、超声心动图、区域血流和整体心肌损伤标志物。通过免疫印迹测定内皮衍生一氧化氮合酶（eNOS）、磷酸化 eNOS（p-eNOS）、丝氨酸/苏氨酸激酶 Akt 总（t-Akt）和磷酸化（p-Akt）的蛋白表达。
所有动物在心肺复苏（CPR）和除颤后均自发恢复循环。预处理动物的血流动力学显著心律失常、心肌顿抑较少，大脑、心脏、肾脏和回肠的区域血流较多。PC 中的肌钙蛋白 I 和肌酸磷酸激酶值为对照值的 65%。此外，预处理动物的心脏 eNOS、p-eNOS、t-Akt 和 p-Akt 的蛋白表达均高于对照。
pGz 预处理在全身缺血再灌注损伤模型中提供早期心脏保护。
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