Indian Veterinary Research Institute, Kolkata, India.
Hum Exp Toxicol. 2010 Nov;29(11):965-72. doi: 10.1177/0960327110364641. Epub 2010 Mar 8.
Oxidative stress due to arsenic toxicity and ameliorative potentiality of L-ascorbic acid was evaluated in an ex vivo system of rat hepatic tissue. The study revealed that arsenic increased the activity of superoxide dismutase (SOD) and catalase (CAT) and the level of lipid peroxidation (LPO), protein carbonyl (PC) and nitric oxide (NO) at 1 hour, 1.5 hours and 2 hours of incubation. Co-treatment with L-ascorbic acid was found effective to normalize the activity of SOD and CAT and the production of LPO, PC and NO in hepatic tissue. This ex vivo study suggested that ascorbic acid is helpful to ameliorate arsenic-induced oxidative stress. This may be one of the alternative screening systems to study the efficacy of antioxidant and hepatoprotective agent.
在大鼠肝组织的离体系统中评估了砷毒性引起的氧化应激和 L-抗坏血酸的改善潜力。研究表明，砷在孵育 1 小时、1.5 小时和 2 小时时增加了超氧化物歧化酶 (SOD) 和过氧化氢酶 (CAT) 的活性以及脂质过氧化 (LPO)、蛋白质羰基 (PC) 和一氧化氮 (NO) 的水平。L-抗坏血酸的共同处理被发现可有效使肝组织中 SOD 和 CAT 的活性以及 LPO、PC 和 NO 的产生正常化。这项离体研究表明，抗坏血酸有助于改善砷引起的氧化应激。这可能是研究抗氧化剂和肝保护剂疗效的替代筛选系统之一。
