Indian Veterinary Research Institute, Eastern Regional Station, Kolkata, India.
Hum Exp Toxicol. 2011 Aug;30(8):940-51. doi: 10.1177/0960327110384521. Epub 2010 Sep 27.
Oyster mushroom, Pleurotus florida is regarded as one of the popular food with biopharmaceutical properties. Here, the study aimed to investigate the antioxidative effects of mushroom (Pleurotus florida) lectin against arsenic-induced nephrotoxicity in rats. Animals were divided into four groups; Group 1 was control. Groups 2, 3 and 4 were exposed to arsenic (20 parts per million [ppm] in drinking water), arsenic plus oral supplementation of ascorbic acid (25 mg/kg body weight) and arsenic plus oral supplementation of mushroom lectin (150 mg/kg body weight) respectively. Both ascorbic acid and mushroom lectin prevented the arsenic-mediated growth retardation and normalized the elevated kidney weight. Disrupted activities of superoxide dismutase (SOD) and catalase (CAT) and enhanced lipid peroxidation (LPO), protein carbonyl (PC) and nitric oxides (NO) production in kidney caused by arsenic could also be maintained towards normalcy by supplementation of mushroom lectin and ascorbic acid. These antioxidative effects were exhibited in a time-dependant manner. Further, arsenic-mediated down-regulation of messenger RNA (mRNA) expression of superoxide dismutase 2 (SOD(2)) gene was obstructed by these agents. Thus it was found that mushroom lectin reversed the effect of arsenic-mediated oxidative stress in a time-dependent manner.
金针菇（Pleurotus florida）被认为是具有生物制药特性的流行食品之一。本研究旨在探讨金针菇凝集素对大鼠砷诱导肾毒性的抗氧化作用。动物分为四组；第 1 组为对照组。第 2、3 和 4 组分别暴露于砷（饮用水中 20 ppm）、砷加口服补充抗坏血酸（25mg/kg 体重）和砷加口服补充金针菇凝集素（150mg/kg 体重）。抗坏血酸和金针菇凝集素均可预防砷引起的生长迟缓，并使升高的肾脏重量正常化。金针菇凝集素和抗坏血酸的补充可维持砷引起的肾脏中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性破坏、脂质过氧化（LPO）、蛋白质羰基（PC）和一氧化氮（NO）产生增加向正常化。这些抗氧化作用呈时间依赖性。此外，这些药物还阻止了砷介导的超氧化物歧化酶 2（SOD(2)）基因的信使 RNA（mRNA）表达下调。因此，金针菇凝集素可在时间依赖性方式下逆转砷介导的氧化应激效应。
