Fan Jian-Ming, Luo Jun, Chen Lu, Teng Man, Bu Dan, Wang Fang-Yu, Wang Li, Wang Chuan-Qing, Zhang Gai-Ping
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002 Zhengzhou, People's Republic of China.
Virus Genes. 2010 Jun;40(3):357-61. doi: 10.1007/s11262-010-0464-9. Epub 2010 Mar 6.
In this study, four strains of Japanese Encephalitis Virus (JEV) were isolated from the cerebrospinal fluid of aborted fetuses or stillborn piglets collected randomly from a number of piggeries in central China. The E genes were cloned by RT-PCR and sequenced. Phylogenetic analysis was performed with 48 JEV isolates previously reported in China and other countries, and showed that all four isolates can be classified into the subcluster of genotype III. The results strongly suggest that the genotype III of JEV is the major variant currently circulating in the swinery of central China.
在本研究中，从中国中部多个养猪场随机采集的流产胎儿或死产仔猪的脑脊液中分离出四株日本脑炎病毒（JEV）。通过RT-PCR克隆并测序E基因。对先前在中国和其他国家报道的48株JEV分离株进行了系统发育分析，结果表明，所有四株分离株均可归类为基因型III的亚群。结果强烈表明，JEV基因型III是目前在中国中部猪群中流行的主要变体。
