Institute of Military Veterinary, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, PR China.
Vector Borne Zoonotic Dis. 2013 Jan;13(1):41-9. doi: 10.1089/vbz.2012.1016. Epub 2012 Nov 30.
The residential regions of Yunnan province, canton of Jing Hong, in China were surveyed for Japanese encephalitis virus (JEV) infection in mosquito and swine vectors to determine the frequency of JEV-carrying zoonotic vectors in 2009-2010. A total of 21,500 mosquitoes were collected and divided by species, and brain tissue was collected from 108 stillborn piglets. The infection rates for the different JEV species were 13.2% for Culex tritaeniorhynchus, 2.7% for Anopheles sinensis, 0.7% for Armigeres subalbatus, and 18.5% for stillborn piglets. The complete genomes of two JEV samples that were collected in different seasons and different regions, Yunnan 0901 and Yunnan 0902, were sequenced from a pool of Culex mosquitoes and stillborn piglets that had been collected randomly from several piggeries. Multiple sequence alignment with 24 fully-sequenced genes and 93 complete sequences of the JEV-encoded E gene revealed nucleotide homologies ranging from 97.2-99.6% and 94.5-99.7% in mosquitoes and piglets, respectively, and deduced amino acid homologies ranging from 97.4-98.1% and 96.0-98.2%, respectively. Phylogenetic analyses of the Yunnan 0901 and Yunnan 0902 strains' full-length genomes and E gene sequences indicated that these strains are most closely related to six Chinese SA14-derived viruses, and distantly related to the Australian FU, vellore P20778, and Japanese Ishikawa strains, and the previously isolated YN86-B8639 strains. The phylogenetic relationships based on the full-length genome were similar to those found for the E gene, indicating that phylogenetic analysis of the E gene will be a useful approach for genotyping of JEV, but not to better understand the potential changes in the biological characteristics and genetic relationship of JEV isolates.
在中国云南省景洪县居民区调查了日本脑炎病毒（JEV）在蚊媒和猪载体中的感染情况，以确定 2009-2010 年 JEV 携带的人畜共患病载体的频率。共采集了 21500 只蚊子，按种类进行了分类，并从 108 头死产仔猪的脑中采集了组织。不同 JEV 种类的感染率分别为：三带喙库蚊 13.2%，中华按蚊 2.7%，白纹伊蚊 0.7%，死产仔猪 18.5%。从不同季节和不同地区随机采集的库蚊和死产仔猪中，对 2 个 JEV 样本（云南 0901 和云南 0902）的完整基因组进行了测序。用 24 个完全测序的基因和 93 个 JEV 编码 E 基因的完整序列与从几个猪圈中随机采集的库蚊和仔猪进行了多重序列比对，结果显示，在蚊子和仔猪中，核苷酸同源性分别为 97.2-99.6%和 94.5-99.7%，推断的氨基酸同源性分别为 97.4-98.1%和 96.0-98.2%。云南 0901 和云南 0902 株全长基因组和 E 基因序列的系统进化分析表明，这些株与 6 株中国的 SA14 衍生病毒最为密切相关，与澳大利亚的 FU、vellore P20778 和日本的 Ishikawa 株以及之前分离的 YN86-B8639 株较远。基于全长基因组的系统进化关系与 E 基因的相似，表明 E 基因的系统进化分析将是 JEV 基因分型的一种有用方法，但不能更好地了解 JEV 分离株的生物学特性和遗传关系的潜在变化。
