从中国猪中分离到的基因型 1 日本脑炎病毒 SX09S-01 的分离和分子特征。
Isolation and molecular characterization of genotype 1 Japanese encephalitis virus, SX09S-01, from pigs in China.
机构信息
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan city 430070, Hubei Province, PR China.
出版信息
Virol J. 2011 Oct 14;8:472. doi: 10.1186/1743-422X-8-472.
BACKGROUND
Pigs play a critical role in Japanese encephalitis virus (JEV) transmission between mosquitos and humans. In 2009, lots of piglets developed symptom of viral encephalitis in a pig farm in Yunchen, Shanxi province.
METHODS
Virus isolation was carried out in BHK-21 cells. Immunohistochemistry, RT-PCR and indirect immunofluorescent assay were used to identify the newly isolated virus. The complete genome of one isolate (SX09S-01 strain) was sequenced and analyzed. Two phylogenetic trees were constructed on the basis of the 24 full-length JEV genomes and 62 E genes mostly selected from China.
RESULTS
JEV SX09S-01 strain was isolated from piglets. Sequence analysis indicates that the completed genome sequences of this strain consists of 10965 nucleotides and there are 13 nucleotides deletion in the 3' nontranslated variable region. Compared with other JEV strains, homology ranges from 99.1% (XJ69) to 74.1% (XZ0934) and 99.6% (XJ69) to 91.1% (XZ0934) on the level of nucleotide and amino acid sequences, respectively. Phylogenetic trees show that SX09S-01 strain belongs to genotype I and it is most closely related to the XJ69 strain.
CONCLUSIONS
Genotype I of JEV still circulates in Yuncheng and it is thus important for active surveillance on genotype I of JEV from the swine population.
背景
猪在日本脑炎病毒（JEV）在蚊子和人类之间的传播中起着至关重要的作用。2009 年，山西省运城市的一个养猪场有许多仔猪出现病毒性脑炎症状。
方法
在 BHK-21 细胞中进行病毒分离。使用免疫组织化学、RT-PCR 和间接免疫荧光检测来鉴定新分离的病毒。对一株分离株（SX09S-01 株）的全基因组进行测序和分析。根据 24 株全长 JEV 基因组和 62 株主要来自中国的 E 基因构建了两个系统进化树。
结果
JEV SX09S-01 株从仔猪中分离得到。序列分析表明，该株的完整基因组序列由 10965 个核苷酸组成，在 3'非翻译可变区有 13 个核苷酸缺失。与其他 JEV 株相比，核苷酸和氨基酸序列的同源性范围分别为 99.1%（XJ69）至 74.1%（XZ0934）和 99.6%（XJ69）至 91.1%（XZ0934）。系统进化树显示，SX09S-01 株属于基因型 I，与 XJ69 株最为密切相关。
结论
基因型 I 的 JEV 仍在运城市流行，因此对猪群中 I 型 JEV 的主动监测非常重要。
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