系统性红斑狼疮患者T细胞上CD44变异体同种型CD44v3和CD44v6的表达增加，且与疾病活动相关。
Expression of CD44 variant isoforms CD44v3 and CD44v6 is increased on T cells from patients with systemic lupus erythematosus and is correlated with disease activity.
作者信息
Crispín José C, Keenan Brendan T, Finnell Michele D, Bermas Bonnie L, Schur Peter, Massarotti Elena, Karlson Elizabeth W, Fitzgerald Lisa M, Ergin Sukran, Kyttaris Vasileios C, Tsokos George C, Costenbader Karen H
机构信息
Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA.
出版信息
Arthritis Rheum. 2010 May;62(5):1431-7. doi: 10.1002/art.27385.
OBJECTIVE
To quantify the expression of CD44 and variant isoforms CD44v3 and CD44v6 on T cells from patients with systemic lupus erythematosus (SLE), and to assess correlations of the level of expression of these molecules with disease manifestations.
METHODS
Information on clinical and demographic characteristics was collected, and blood samples were obtained from 72 patients with SLE and 32 healthy control subjects matched to the patients by sex, race, and age. Expression of CD44 and variants CD44v3 and v6 on T cell subsets was determined by flow cytometry, and Pearson's correlations of their expression levels with clinical variables, SLE Disease Activity Index (SLEDAI) scores, and presence of lupus nephritis were determined. Wilcoxon's rank sum tests and conditional multivariable regression analyses were applied to identify differences in the expression of CD44 between patients with SLE and healthy controls.
RESULTS
Expression of CD44 was higher on CD4+ and CD8+ T cells from SLE patients compared with controls (P <or= 0.03). Expression of CD44v3 and CD44v6 was also higher on total T cells and CD4+ and CD8+ T cells from SLE patients compared with controls (P <or= 0.03). Cell surface levels of CD44v3 on total T cells, CD4+ T cells, and CD8+ T cells as well as cell surface expression of CD44v6 on total T cells and CD4+ T cells were correlated with the SLEDAI score (P < 0.05). The presence of lupus nephritis was associated with the expression of CD44v6 on total T cells, CD4+ T cells, and CD4-CD8- T cells (P < 0.05). Positivity for anti-double-stranded DNA antibodies was associated with the expression levels of CD44v6 on T cells (P < 0.05).
CONCLUSION
These results indicate that expression levels of CD44v3 and CD44v6 on T cells may represent useful biomarkers of SLE activity.
目的
量化系统性红斑狼疮（SLE）患者T细胞上CD44及其变异体CD44v3和CD44v6的表达，并评估这些分子的表达水平与疾病表现的相关性。
方法
收集临床和人口统计学特征信息，从72例SLE患者和32名按性别、种族和年龄匹配的健康对照者中采集血样。通过流式细胞术测定T细胞亚群上CD44及其变异体CD44v3和v6的表达，并确定其表达水平与临床变量、SLE疾病活动指数（SLEDAI）评分以及狼疮肾炎的存在情况之间的Pearson相关性。应用Wilcoxon秩和检验和条件多变量回归分析来确定SLE患者与健康对照者之间CD44表达的差异。
结果
与对照组相比，SLE患者CD4+和CD8+T细胞上CD44的表达更高（P≤0.03）。与对照组相比，SLE患者总T细胞、CD4+和CD8+T细胞上CD44v3和CD44v6的表达也更高（P≤0.03）。总T细胞、CD4+T细胞和CD8+T细胞上CD44v3的细胞表面水平以及总T细胞和CD4+T细胞上CD44v6的细胞表面表达与SLEDAI评分相关（P<0.05）。狼疮肾炎的存在与总T细胞、CD4+T细胞和CD4-CD8-T细胞上CD44v6的表达相关（P<0.05）。抗双链DNA抗体阳性与T细胞上CD44v6的表达水平相关（P<0.05）。
结论
这些结果表明，T细胞上CD44v3和CD44v6的表达水平可能代表SLE活动的有用生物标志物。
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