Brady A J, Farnan T B, Toner J G, Gilpin D F, Tunney M M
Clinical and Practice Research Group, School of Pharmacy, Queen's University Belfast Medical Biology Centre, Northern Ireland, UK.
J Laryngol Otol. 2010 Jul;124(7):729-38. doi: 10.1017/S0022215110000319. Epub 2010 Mar 10.
This study aimed to investigate antimicrobial treatment of an infected cochlear implant, undertaken in an attempt to salvage the infected device.
We used the broth microdilution method to assess the susceptibility of meticillin-sensitive Staphylococcus aureus isolate, cultured from an infected cochlear implant, to common antimicrobial agents as well as to novel agents such as tea tree oil. To better simulate in vivo conditions, where bacteria grow as microcolonies encased in glycocalyx, the bactericidal activity of selected antimicrobial agents against the isolate growing in biofilm were also compared.
When grown planktonically, the S aureus isolate was susceptible to 17 of the 18 antimicrobials tested. However, when grown in biofilm, it was resistant to all conventional antimicrobials. In contrast, 5 per cent tea tree oil completely eradicated the biofilm following exposure for 1 hour.
Treatment of infected cochlear implants with novel agents such as tea tree oil could significantly improve salvage outcome.
本研究旨在调查对感染的人工耳蜗进行抗菌治疗，以挽救受感染的装置。
我们采用肉汤微量稀释法评估从感染的人工耳蜗培养出的耐甲氧西林金黄色葡萄球菌分离株对常见抗菌药物以及茶树油等新型药物的敏感性。为了更好地模拟体内条件，即细菌以包裹在糖萼中的微菌落形式生长，还比较了所选抗菌药物对生物膜中生长的分离株的杀菌活性。
当金黄色葡萄球菌分离株浮游生长时，对所测试的18种抗菌药物中的17种敏感。然而，当在生物膜中生长时，它对所有传统抗菌药物均耐药。相比之下，5%的茶树油在暴露1小时后可完全清除生物膜。
用茶树油等新型药物治疗感染的人工耳蜗可显著改善挽救效果。
