Department of Public Health, University of Copenhagen, Koebenhavn K, Denmark.
Reprod Toxicol. 2010 Aug;30(1):131-7. doi: 10.1016/j.reprotox.2010.02.007. Epub 2010 Mar 7.
Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125 microM. BPA exposure significantly increased beta-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1 nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.
双酚 A（BPA）是一种类雌激素化学物质，会从消费品中渗出，从而可能导致人体接触。为了研究妊娠期间 BPA 暴露的影响，我们使用 BeWo 滋养层细胞系、胎盘组织培养物、胎盘灌注和皮肤扩散模型进行了研究，这些模型均来自人体。结果表明，BPA 对 BeWo 细胞具有细胞毒性，其表观 EC50 为 100-125μM。在环境相关浓度 1 nM 下，BPA 暴露显著增加了胎盘组织培养物中β-hCG 的分泌和 caspase-3 的表达。在转运研究中，BPA 迅速穿过足月胎盘和 BeWo 细胞单层进行转运。此外，还观察到 BPA 的经皮转运。这些结果表明，胎儿通过胎盘交换暴露于 BPA 可能对胎盘和胎儿发育产生不利影响。这一系列在人类着床和胎儿发育领域的测试系统代表了生殖毒性风险评估中的重要元素。
