Bioresource Collection and Research Center, Food Industry Research and Development Institute, National Chiao Tung University, Hsinchu, Taiwan, ROC.
Cells Tissues Organs. 2010;192(2):93-105. doi: 10.1159/000295774. Epub 2010 Mar 9.
The amniotic membrane has been clinically applied as a therapeutic material in wound covering and corneal surface reconstruction. Recently, mesenchymal stem cells (MSCs) have been isolated from the placenta, specifically from the amniotic membrane. However, the localization of MSCs in the amniotic membrane has not been determined. In this study, term placenta was collected, and we performed immunohistochemical staining techniques to identify and localize MSCs in the mesoderm of the amniotic membrane in situ with MSC antibodies, including CD90 and CD105. We further directly cultured and characterized MSCs from the amniotic membrane mesoderm (AMSCs). The AMSCs were easily isolated and represented a homogenous fibroblastic morphology at early passages. In addition to MSC surface markers, AMSCs expressed Sox2, Oct-4 and Nanog. AMSCs could be induced into osteocytes, adipocytes and chondrocytes in vitro and show immunosuppressive effects on T-cell proliferation. Under appropriate conditions, AMSCs could differentiate into neuronal-like cells, which were identified by neuronal-specific markers and their ability to secrete dopamine. This study reveals that AMSCs provide a promising source for stem cell studies and also extend the clinical potential of the amniotic membrane in the field of regenerative medicine.
羊膜已被临床应用于创伤覆盖和角膜表面重建的治疗材料。最近，间充质干细胞（MSCs）已从胎盘，特别是从羊膜中分离出来。然而，MSCs 在羊膜中的定位尚未确定。在这项研究中，我们收集了足月胎盘，并使用 MSC 抗体（包括 CD90 和 CD105）进行免疫组织化学染色技术，以原位鉴定和定位羊膜中胚层中的 MSCs。我们进一步直接从羊膜中胚层（AMSCs）培养和表征 MSCs。AMSCs 很容易分离，在早期传代时呈现出均一的成纤维细胞形态。除了 MSC 表面标志物外，AMSCs 还表达 Sox2、Oct-4 和 Nanog。AMSCs 可以在体外诱导为成骨细胞、脂肪细胞和成软骨细胞，并对 T 细胞增殖具有免疫抑制作用。在适当的条件下，AMSCs 可以分化为神经元样细胞，这些细胞可以通过神经元特异性标志物及其分泌多巴胺的能力来识别。这项研究表明，AMSCs 为干细胞研究提供了有前途的来源，并扩展了羊膜在再生医学领域的临床潜力。
