Vidane Atanasio S, Souza Aline F, Sampaio Rafael V, Bressan Fabiana F, Pieri Naira C, Martins Daniele S, Meirelles Flavio V, Miglino Maria A, Ambrósio Carlos E
Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, São Paulo, Brazil.
Stem Cells Cloning. 2014 Aug 27;7:71-8. doi: 10.2147/SCCAA.S67790. eCollection 2014.
Amnion-derived mesenchymal stem cells (AMSCs) are multipotent cells with an enhanced ability to differentiate into multiple lineages. AMSCs can be acquired through noninvasive methods, and therefore are exempt from the typical ethical issues surrounding stem cell use. The objective of this study was to isolate and characterize AMSCs from a cat amniotic membrane for future application in regenerative medicine. The cat AMSCs were harvested after mechanical and enzymatic digestion of amnion. In culture medium, the cat AMSCs adhered to a plastic culture dish and displayed a fibroblast-like morphology. Immunophenotyping assays were positive for the mesenchymal stem cell-specific markers CD73 and CD90 but not the hematopoietic markers CD34, CD45, and CD79. Under appropriate conditions, the cat AMSCs differentiated into osteogenic, chondrogenic, and adipogenic cell lineages. One advantage of cat AMSCs was nonteratogenicity, assessed 4 weeks post injection of undifferentiated AMSCs into immunodeficient mice. These findings suggest that cat amniotic membranes may be an important and useful source of mesenchymal stem cells for clinical applications, especially for cell or tissue replacement in chronic and degenerative diseases.
羊膜来源的间充质干细胞（AMSCs）是具有增强的多向分化能力的多能细胞。AMSCs可以通过非侵入性方法获取，因此不受干细胞使用中典型伦理问题的影响。本研究的目的是从猫羊膜中分离和鉴定AMSCs，以便将来应用于再生医学。猫AMSCs是在对羊膜进行机械和酶消化后收获的。在培养基中，猫AMSCs附着于塑料培养皿并呈现成纤维细胞样形态。免疫表型分析显示间充质干细胞特异性标志物CD73和CD90呈阳性，但造血标志物CD34、CD45和CD79呈阴性。在适当条件下，猫AMSCs可分化为成骨、成软骨和脂肪生成细胞系。猫AMSCs的一个优点是无致畸性，这是在将未分化的AMSCs注射到免疫缺陷小鼠中4周后评估得出的。这些发现表明，猫羊膜可能是临床应用中重要且有用的间充质干细胞来源，特别是用于慢性和退行性疾病的细胞或组织替代。
