Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy.
Pharmacology. 2010;85(4):195-202. doi: 10.1159/000275063. Epub 2010 Mar 6.
The aim of this study was to explore the antiradical activity of Met I (an active metabolite of erdosteine) containing a pharmacologically active sulphydryl group, by means of electron paramagnetic resonance (EPR) spectroscopy which has not previously been used to characterize the antiradical activity of Met I. The effects of concentrations of 20, 10, 5, 2.5, 1.25 and 0.625 microg/ml of Met I were tested against: (a) the Fenton reaction model system with EPR detection of HO.; (b) the KO2-crown ether system with EPR detection of O2-.; (c) the EPR assay based on the reduction of the Tempol radical, and (d) the EPR assay based on the reduction of Fremy's salt radical. Our findings show that the intensity of 4 different free radicals was significantly reduced in the presence of Met I, thus indicating the presence of a termination reaction between the free radicals and Met I.
本研究旨在通过电子顺磁共振（EPR）光谱法探索含有药理活性巯基的Met I（厄多司坦的一种活性代谢物）的抗自由基活性，EPR 光谱法以前尚未用于表征 Met I 的抗自由基活性。测试了浓度为 20、10、5、2.5、1.25 和 0.625μg/ml 的 Met I 对以下方面的影响：（a）通过 EPR 检测 HO.的 Fenton 反应模型系统；（b）通过 EPR 检测 O2-的 KO2-冠醚系统；（c）基于 Tempol 自由基还原的 EPR 测定法，以及（d）基于 Fremy 盐自由基还原的 EPR 测定法。我们的研究结果表明，在 Met I 的存在下，4 种不同自由基的强度显著降低，这表明自由基与 Met I 之间存在终止反应。
