University of Primorska, College of Health Care Izola, Izola.
Melanoma Res. 2011 Jun;21(3):196-205. doi: 10.1097/CMR.0b013e328337abd7.
Electrochemotherapy is a local treatment combining chemotherapy and application of electric pulses to the tumour. Electrochemotherapy with bleomycin and cisplatin has shown its effectiveness in controlling local tumour growth in the treatment of malignant melanoma. However, the effect of electrochemotherapy on the metastatic potential of tumour cells is not known. Prevention of metastasis is an important aspect of successful treatment; however, it is known that metastasis can be induced by different treatment modalities. Therefore, the aim of this study was to evaluate the effect of electrochemotherapy with cisplatin on the metastatic potential of human malignant melanoma cells. Cells treated by electrochemotherapy with cisplatin were tested for their ability to migrate and invade through Matrigel-coated porous membrane. In addition, RNA was isolated from cells after treatment and differentially expressed genes were investigated by microarray analysis to evaluate the effect of electrochemotherapy with cisplatin on gene expression. There were no significant changes observed in cell migration and invasion of melanoma cells after electrochemotherapy. In addition, there were no changes observed in cell adhesion on Matrigel. Gene expression analysis showed that a very low number of genes were differentially expressed after electrochemotherapy with cisplatin. Two genes, LAMB3 and CD63 involved in cell migration, were both downregulated after electrochemotherapy with cisplatin and the expression of metastasis promoting genes was not increased after electrochemotherapy. Our data suggest that electrochemotherapy does not increase the metastatic behaviour of human melanoma cells.
电化学疗法是一种将化疗与肿瘤电脉冲应用相结合的局部治疗方法。博来霉素和顺铂联合电化学疗法在控制恶性黑色素瘤的局部肿瘤生长方面已显示出其疗效。然而，电化学疗法对肿瘤细胞转移潜能的影响尚不清楚。预防转移是成功治疗的一个重要方面；然而，众所周知，不同的治疗方式都可能诱导转移。因此，本研究旨在评估顺铂电化学疗法对人恶性黑色素瘤细胞转移潜能的影响。通过 Matrigel 包被的多孔膜检测经顺铂电化学疗法处理的细胞的迁移和侵袭能力。此外，还从处理后的细胞中分离 RNA，并通过微阵列分析研究差异表达基因，以评估顺铂电化学疗法对基因表达的影响。在顺铂电化学疗法后，黑色素瘤细胞的迁移和侵袭能力没有明显变化。此外，在 Matrigel 上的细胞黏附也没有观察到变化。基因表达分析表明，在顺铂电化学疗法后仅有极少数基因表达发生差异。两个参与细胞迁移的基因 LAMB3 和 CD63 在顺铂电化学疗法后均下调，并且在顺铂电化学疗法后促进转移的基因表达没有增加。我们的数据表明，电化学疗法不会增加人黑色素瘤细胞的转移行为。
