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评估博来霉素电化学疗法后存活的 SK-MEL28 黑素瘤细胞的致瘤性和转移性。

Assessment of the tumourigenic and metastatic properties of SK-MEL28 melanoma cells surviving electrochemotherapy with bleomycin.

机构信息

Institute of Oncology Ljubljana, Department of Experimental Oncology, Ljubljana, Slovenia.

出版信息

Radiol Oncol. 2012 Mar;46(1):32-45. doi: 10.2478/v10019-012-0010-6. Epub 2012 Jan 12.

DOI:10.2478/v10019-012-0010-6
PMID:22933978
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3423760/
Abstract

BACKGROUND

Electrochemotherapy is a local treatment combining chemotherapy and electroporation and is highly effective treatment approach for subcutaneous tumours of various histologies. Contrary to surgery and radiation, the effect of electrochemotherapy on metastatic potential of tumour cells has not been extensively studied. The aim of the study was to evaluate the effect of electrochemotherapy with bleomycin on the metastatic potential of human melanoma cells in vitro.

MATERIALS AND METHODS

Viable cells 48 hours after electrochemotherapy were tested for their ability to migrate and invade through Matrigel coated porous membrane. In addition, microarray analysis and quantitative Real-Time PCR were used to detect changes in gene expression after electrochemotherapy.

RESULTS

Cell migration and invasion were not changed in melanoma cells surviving electrochemotherapy. Interestingly, only a low number of tumourigenesis related genes was differentially expressed after electrochemotherapy.

CONCLUSIONS

Our data suggest that metastatic potential of human melanoma cells is not affected by electrochemotherapy with bleomycin, confirming safe role of electrochemotherapy in the clinics.

摘要

背景

电化疗是一种将化疗和电穿孔相结合的局部治疗方法，对各种组织学来源的皮下肿瘤具有高度有效性。与手术和放疗不同，电化疗对肿瘤细胞转移潜能的影响尚未得到广泛研究。本研究旨在评估电化疗联合博来霉素对体外人黑色素瘤细胞转移潜能的影响。

材料和方法

电化学治疗后 48 小时，检测活细胞穿过 Matrigel 包被的多孔膜的迁移和侵袭能力。此外，还使用微阵列分析和定量实时 PCR 检测电化学治疗后基因表达的变化。

结果

电化学治疗后存活的黑色素瘤细胞的迁移和侵袭能力没有改变。有趣的是，电化学治疗后只有少数与肿瘤发生相关的基因表达发生差异。

结论

我们的数据表明，电化疗联合博来霉素对人黑色素瘤细胞的转移潜能没有影响，这证实了电化疗在临床应用中的安全性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a6/3423760/d96f62edd7b4/rado-46-01-32f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a6/3423760/45190fa6ab1c/rado-46-01-32f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a6/3423760/25c9e640b0e7/rado-46-01-32f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a6/3423760/69fed9ef4480/rado-46-01-32f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a6/3423760/d96f62edd7b4/rado-46-01-32f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a6/3423760/45190fa6ab1c/rado-46-01-32f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a6/3423760/25c9e640b0e7/rado-46-01-32f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a6/3423760/69fed9ef4480/rado-46-01-32f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a6/3423760/d96f62edd7b4/rado-46-01-32f4.jpg

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Role of radiotherapy in melanoma management.放疗在黑色素瘤治疗中的作用。
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Tracing the origins of metastasis.追踪转移的起源。
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Staurosporine induces different cell death forms in cultured rat astrocytes.星孢菌素诱导培养的大鼠星形胶质细胞产生不同的细胞死亡形式。
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Electrochemotherapy for cutaneous and subcutaneous tumor lesions: a novel therapeutic approach.电化学疗法治疗皮肤和皮下肿瘤病变：一种新的治疗方法。
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Plasmid injection and application of electric pulses alter endogenous mRNA and protein expression in B16.F10 mouse melanomas.质粒注射和电脉冲应用改变了 B16.F10 小鼠黑色素瘤中的内源性 mRNA 和蛋白质表达。
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